Title |
Subtractive assembly for comparative metagenomics, and its application to type 2 diabetes metagenomes
|
---|---|
Published in |
Genome Biology, November 2015
|
DOI | 10.1186/s13059-015-0804-0 |
Pubmed ID | |
Authors |
Mingjie Wang, Thomas G. Doak, Yuzhen Ye |
Abstract |
Comparative metagenomics remains challenging due to the size and complexity of metagenomic datasets. Here we introduce subtractive assembly, a de novo assembly approach for comparative metagenomics that directly assembles only the differential reads that distinguish between two groups of metagenomes. Using simulated datasets, we show it improves both the efficiency of the assembly and the assembly quality of the differential genomes and genes. Further, its application to type 2 diabetes (T2D) metagenomic datasets reveals clear signatures of the T2D gut microbiome, revealing new phylogenetic and functional features of the gut microbial communities associated with T2D. |
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Germany | 2 | 8% |
Canada | 1 | 4% |
Luxembourg | 1 | 4% |
Spain | 1 | 4% |
Taiwan | 1 | 4% |
Unknown | 6 | 25% |
Demographic breakdown
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Scientists | 13 | 54% |
Members of the public | 10 | 42% |
Science communicators (journalists, bloggers, editors) | 1 | 4% |
Mendeley readers
Geographical breakdown
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Canada | 3 | 3% |
Germany | 2 | 2% |
Brazil | 2 | 2% |
Argentina | 1 | <1% |
Japan | 1 | <1% |
Spain | 1 | <1% |
Unknown | 86 | 83% |
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Researcher | 24 | 23% |
Student > Ph. D. Student | 18 | 17% |
Student > Master | 16 | 16% |
Other | 9 | 9% |
Professor | 7 | 7% |
Other | 21 | 20% |
Unknown | 8 | 8% |
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Biochemistry, Genetics and Molecular Biology | 20 | 19% |
Computer Science | 11 | 11% |
Medicine and Dentistry | 9 | 9% |
Immunology and Microbiology | 3 | 3% |
Other | 5 | 5% |
Unknown | 11 | 11% |