Title |
Autologous immuno magnetically selected CD133+ stem cells in the treatment of no-option critical limb ischemia: clinical and contrast enhanced ultrasound assessed results in eight patients
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Published in |
Journal of Translational Medicine, November 2015
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DOI | 10.1186/s12967-015-0697-4 |
Pubmed ID | |
Authors |
Vittorio Arici, Cesare Perotti, Calliada Fabrizio, Claudia Del Fante, Franco Ragni, Francesco Alessandrino, Gianluca Viarengo, Michele Pagani, Alessia Moia, Carmine Tinelli, Antonio Bozzani |
Abstract |
Demonstrate the safety and effectiveness of highly purified CD133+ autologous stem cells in critical limb ischemia (CLI). Prospective single-center not randomized. Clinicaltrials.gov identifier: NCT01595776 METHODS: Eight patients with a history of stable CLI were enrolled in a period of 2 years. After bone marrow stimulation and single leukapheresis collection, CD133+ immunomagnetic cell selection was performed. CD133+ cells in buffer phosphate suspension was administered intramuscularly. Muscular and arterial contrast enhanced ultra sound (CEUS), lesion evolution and pain management were assessed preoperatively and 3, 6 and 12 months after the implant. No patient had early or late complications related to the procedure. Two patients (25 %) didn't get any relief from the treatment and underwent major amputation. Six patients (75 %) had a complete healing of the wounds, rest pain cessation and walking recovery. An increase in CEUS values was shown in all eight patients at 6 months and in the six clinical healed patients at 12 months and had statistical relevance. Highly purified autologous CD133+ cells can stimulate neo-angiogenesis, as based on clinical and CEUS data. |
X Demographics
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Unknown | 3 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 1 | 1% |
Germany | 1 | 1% |
Unknown | 66 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 10 | 15% |
Student > Bachelor | 10 | 15% |
Other | 9 | 13% |
Researcher | 5 | 7% |
Student > Master | 4 | 6% |
Other | 11 | 16% |
Unknown | 19 | 28% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 19 | 28% |
Nursing and Health Professions | 8 | 12% |
Agricultural and Biological Sciences | 5 | 7% |
Biochemistry, Genetics and Molecular Biology | 4 | 6% |
Psychology | 4 | 6% |
Other | 7 | 10% |
Unknown | 21 | 31% |