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Neutralizing antibodies against porcine epidemic diarrhea virus block virus attachment and internalization

Overview of attention for article published in Virology Journal, August 2018
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Title
Neutralizing antibodies against porcine epidemic diarrhea virus block virus attachment and internalization
Published in
Virology Journal, August 2018
DOI 10.1186/s12985-018-1042-3
Pubmed ID
Authors

Lang Gong, Ying Lin, Jianru Qin, Qianniu Li, Chunyi Xue, Yongchang Cao

Abstract

Porcine epidemic diarrhea virus (PEDV) is emerging as a pathogenic coronavirus that causes a huge economic burden to the swine industry. Interaction of the viral spike (S) surface glycoprotein with the host cell receptor is recognized as the first step of infection and is the main determinant of virus tropism. The mechanisms by which neutralizing antibodies inhibit PEDV have not been defined. Isolating PEDV neutralizing antibodies are crucial to identifying the receptor-binding domains of the viral spike and elucidating the mechanism of protection against PEDV infection. B cell hybridoma technique was used to generate hybridoma cells that secrete specific antibodies. E.coli prokaryotic expression system and Bac-to-Bac expression system were used to identify the target protein of each monoclonal antibody. qPCR was performed to analyze PEDV binding to Vero E6 cells with neutralizing antibody. We identified 10 monoclonal antibodies using hybridoma technology. Remarkably, 4 mAbs (designed 2G8, 2B11, 3D9, 1E3) neutralized virus infection potently, of which 2B11 and 1E3 targeted the conformational epitope of the PEDV S protein. qPCR results showed that both 2B11 and 2G8 blocked virus entry into Vero cells. The data suggested that PEDV neutralizing antibody inhibited virus infection by binding to infectious virions, which could work as a tool to find the receptor-binding domains.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 29 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 21%
Student > Master 5 17%
Student > Ph. D. Student 4 14%
Other 2 7%
Librarian 2 7%
Other 6 21%
Unknown 4 14%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 8 28%
Biochemistry, Genetics and Molecular Biology 5 17%
Agricultural and Biological Sciences 4 14%
Arts and Humanities 1 3%
Business, Management and Accounting 1 3%
Other 4 14%
Unknown 6 21%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 August 2018.
All research outputs
#10,701,187
of 13,451,706 outputs
Outputs from Virology Journal
#1,716
of 2,188 outputs
Outputs of similar age
#199,268
of 266,292 outputs
Outputs of similar age from Virology Journal
#1
of 1 outputs
Altmetric has tracked 13,451,706 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,188 research outputs from this source. They receive a mean Attention Score of 3.5. This one is in the 7th percentile – i.e., 7% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them