Title |
An explorative study on deep profiling of peripheral leukocytes to identify predictors for responsiveness to anti-tumour necrosis factor alpha therapies in ankylosing spondylitis: natural killer cells in focus
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Published in |
Arthritis Research & Therapy, August 2018
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DOI | 10.1186/s13075-018-1692-y |
Pubmed ID | |
Authors |
Ursula Schulte-Wrede, Till Sörensen, Joachim R. Grün, Thomas Häupl, Heike Hirseland, Marta Steinbrich-Zöllner, Peihua Wu, Andreas Radbruch, Denis Poddubnyy, Joachim Sieper, Uta Syrbe, Andreas Grützkau |
Abstract |
Therapeutic targeting of tumour necrosis factor (TNF)-α is highly effective in ankylosing spondylitis (AS) patients. However, since one-third of anti-TNF-treated AS patients do not show an adequate clinical response there is an urgent need for new biomarkers that would aid clinicians in their decision-making to select appropriate therapeutic options. Thus, the aim of this explorative study was to identify cell-based biomarkers in peripheral blood that could be used for a pre-treatment stratification of AS patients. A high-dimensional, multi-parametric flow cytometric approach was applied to identify baseline predictors in 31 AS patients before treatment with the TNF blockers adalimumab (TNF-neutralisation) and etanercept (soluble TNF receptor). As the major result, the frequencies of natural killer (NK) cells, and in particular CD8-positive (CD8+) NK cell subsets, were most predictive for therapeutic outcome in AS patients. While an inverse correlation between classical CD56+/CD16+ NK cells and reduction of disease activity was observed, the CD8+ NK cell subset behaved in the opposite direction. At baseline, responders showed significantly increased frequencies of CD8+ NK cells compared with non-responders. This is the first study demonstrating that the composition of the NK cell compartment has predictive power for prediction of therapeutic outcome for anti-TNF-α blockers, and we identified CD8+ NK cells as a potential new player in the TNF-α-driven chronic inflammatory immune response of AS. |
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United States | 1 | 33% |
Unknown | 2 | 67% |
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Members of the public | 3 | 100% |
Mendeley readers
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Student > Master | 5 | 19% |
Student > Ph. D. Student | 3 | 12% |
Other | 2 | 8% |
Student > Postgraduate | 2 | 8% |
Lecturer | 1 | 4% |
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Immunology and Microbiology | 2 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
Unspecified | 1 | 4% |
Other | 4 | 15% |
Unknown | 10 | 38% |