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The therapeutic potential of bacteriophages targeting gram-negative bacteria using Galleria mellonella infection model

Overview of attention for article published in BMC Microbiology, August 2018
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (85th percentile)

Mentioned by

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22 tweeters

Citations

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46 Dimensions

Readers on

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128 Mendeley
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Title
The therapeutic potential of bacteriophages targeting gram-negative bacteria using Galleria mellonella infection model
Published in
BMC Microbiology, August 2018
DOI 10.1186/s12866-018-1234-4
Pubmed ID
Authors

Prasanth Manohar, Ramesh Nachimuthu, Bruno S. Lopes

Abstract

Phage therapy is the therapeutic use of bacteriophages to treat highly drug resistant bacterial infections. The current surge in bacteriophage therapy is motivated mainly because of the emergence of antibiotic-resistant bacteria in clinics. This study evaluated the therapeutic potential of three bacteriophages isolated against Escherichia coli ec311, Klebsiella pneumoniae kp235 and Enterobacter cloacae el140 strains using Galleria mellonella. The in vitro activity of three different phages belonging to Podoviridae and Myoviridae families was studied by the double agar overlay method against multi-drug resistant strains. Larval survivability studies were performed to evaluate the potential of phages against infection using G. mellonella. All the three phages were found to have potential to infect the host bacterial strains. For in vivo studies it was observed that E. coli and E. cloacae infected larvae, should be treated with three phage doses (20 μL, 104 PFU/mL) at 6 h interval to achieve 100% survival rate. But in the case of K. pneumoniae, a single phage dose treatment showed promising outcome. When mixed bacterial infections (all three bacterial cultures at 108 CFU/mL) were tested, minimum of four doses of phage cocktail (three phages) at 6 h interval was necessary to recover the larvae. All the results were confirmed by enumerating bacteria from the larvae. Our data shows that although in vitro studies showed high infectivity of phages, for in vivo models multiple phage doses were required for effective treatment.

Twitter Demographics

The data shown below were collected from the profiles of 22 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 128 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 128 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 24 19%
Student > Master 20 16%
Student > Bachelor 19 15%
Researcher 13 10%
Student > Doctoral Student 9 7%
Other 15 12%
Unknown 28 22%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 29 23%
Immunology and Microbiology 24 19%
Agricultural and Biological Sciences 18 14%
Medicine and Dentistry 9 7%
Veterinary Science and Veterinary Medicine 5 4%
Other 10 8%
Unknown 33 26%

Attention Score in Context

This research output has an Altmetric Attention Score of 14. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 October 2019.
All research outputs
#2,007,749
of 21,510,091 outputs
Outputs from BMC Microbiology
#140
of 3,060 outputs
Outputs of similar age
#42,812
of 296,674 outputs
Outputs of similar age from BMC Microbiology
#1
of 1 outputs
Altmetric has tracked 21,510,091 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,060 research outputs from this source. They receive a mean Attention Score of 4.0. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 296,674 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 85% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them