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Tumor-associated macrophages derived CCL18 promotes metastasis in squamous cell carcinoma of the head and neck

Overview of attention for article published in Cancer Cell International, August 2018
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Title
Tumor-associated macrophages derived CCL18 promotes metastasis in squamous cell carcinoma of the head and neck
Published in
Cancer Cell International, August 2018
DOI 10.1186/s12935-018-0620-1
Pubmed ID
Authors

Li She, Yuexiang Qin, Juncheng Wang, Chao Liu, Gangcai Zhu, Guo Li, Ming Wei, Changhan Chen, Guancheng Liu, Diekuo Zhang, Xiyu Chen, Yunyun Wang, Yuanzheng Qiu, Yongquan Tian, Xin Zhang, Yong Liu, Donghai Huang

Abstract

Alternatively activated macrophages in tumor microenvironment is defined as M2 tumor-associated macrophages (M2 TAMs) that promote cancer progression. However, communicative mechanisms between M2 TAMs and cancer cells in squamous cell carcinoma of head and neck (SCCHN) remain largely unknown. Quantitative real-time PCR, western blotting, enzyme-linked immunosorbent assay and flow cytometry were applied to quantify mRNA and protein expression of genes related to M2 TAMs, epithelial-mesenchymal transition (EMT) and stemness. Wounding-healing and Transwell invasion assays were performed to detect the invasion and migration. Sphere formation assay was used to detect the stemness of SCCHN cells. RNA-sequencing and following bioinformatics analysis were used to determine the alterations of transcriptome. THP-1 monocytes were successfully polarized into M2-like TAMs, which was manifested by increased mRNA and protein expression of CCL18, IL-10 and CD206. Conditioned medium from M2-like TAMs promoted the migration and invasion of SCCHN cells, which was accompanied by the occurrence of EMT and enhanced stemness. Importantly, CCL18 neutralizing antibody partially abrogated these effects that caused by conditional medium from M2-like TAMs. In addition, recombinant human CCL18 (rhCCL18) correspondingly promoted the malignant biological behaviors of SCCHN in vitro. Finally, RNA-sequencing analysis identified 331 up-regulated and 363 down-regulated genes stimulated by rhCCL18, which were statistically enriched in 10 cancer associated signaling pathways. These findings indicate that CCL18 derived from M2-like TAMs promotes metastasis via inducing EMT and cancer stemness in SCCHN in vitro.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 26 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 6 23%
Student > Master 5 19%
Researcher 3 12%
Student > Doctoral Student 2 8%
Student > Ph. D. Student 2 8%
Other 4 15%
Unknown 4 15%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 35%
Medicine and Dentistry 5 19%
Immunology and Microbiology 4 15%
Agricultural and Biological Sciences 1 4%
Pharmacology, Toxicology and Pharmaceutical Science 1 4%
Other 0 0%
Unknown 6 23%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 September 2018.
All research outputs
#11,949,878
of 13,477,526 outputs
Outputs from Cancer Cell International
#411
of 562 outputs
Outputs of similar age
#228,815
of 265,471 outputs
Outputs of similar age from Cancer Cell International
#1
of 1 outputs
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So far Altmetric has tracked 562 research outputs from this source. They receive a mean Attention Score of 2.6. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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