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De novo assembly of the dual transcriptomes of a polymorphic raptor species and its malarial parasite

Overview of attention for article published in BMC Genomics, December 2015
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  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (77th percentile)
  • High Attention Score compared to outputs of the same age and source (85th percentile)

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Title
De novo assembly of the dual transcriptomes of a polymorphic raptor species and its malarial parasite
Published in
BMC Genomics, December 2015
DOI 10.1186/s12864-015-2254-1
Pubmed ID
Authors

Martina Pauli, Nayden Chakarov, Oliver Rupp, Jörn Kalinowski, Alexander Goesmann, Michael D. Sorenson, Oliver Krüger, Joseph Ivan Hoffman

Abstract

Studies of non-model species are important for understanding the molecular processes underpinning phenotypic variation under natural ecological conditions. The common buzzard (Buteo buteo; Aves: Accipitriformes) is a widespread and common Eurasian raptor with three distinct plumage morphs that differ in several fitness-related traits, including parasite infestation. To provide a genomic resource for plumage polymorphic birds in general and to search for candidate genes relating to fitness, we generated a transcriptome from a single dead buzzard specimen plus easily accessible, minimally invasive samples from live chicks. We not only de novo assembled a near-complete buzzard transcriptome, but also obtained a significant fraction of the transcriptome of its malaria-like parasite, Leucocytozoon buteonis. By identifying melanogenesis-related transcripts that are differentially expressed in light ventral and dark dorsal feathers, but which are also expressed in other regions of the body, we also identified a suite of candidate genes that could be associated with fitness differences among the morphs. These include several immune-related genes, providing a plausible link between melanisation and parasite load. qPCR analysis of a subset of these genes revealed significant differences between ventral and dorsal feathers and an additional effect of morph. This new resource provides preliminary insights into genes that could be involved in fitness differences between the buzzard colour morphs, and should facilitate future studies of raptors and their malaria-like parasites.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 58 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 2%
United States 1 2%
Netherlands 1 2%
Germany 1 2%
Unknown 54 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 10 17%
Researcher 10 17%
Student > Master 7 12%
Student > Doctoral Student 6 10%
Student > Bachelor 5 9%
Other 10 17%
Unknown 10 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 23 40%
Environmental Science 6 10%
Biochemistry, Genetics and Molecular Biology 5 9%
Computer Science 4 7%
Medicine and Dentistry 2 3%
Other 5 9%
Unknown 13 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 August 2023.
All research outputs
#5,419,989
of 25,353,525 outputs
Outputs from BMC Genomics
#2,150
of 11,226 outputs
Outputs of similar age
#84,282
of 402,232 outputs
Outputs of similar age from BMC Genomics
#50
of 342 outputs
Altmetric has tracked 25,353,525 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 11,226 research outputs from this source. They receive a mean Attention Score of 4.8. This one has done well, scoring higher than 79% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 402,232 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 77% of its contemporaries.
We're also able to compare this research output to 342 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 85% of its contemporaries.