Title |
IL-32γ attenuates airway fibrosis by modulating the integrin-FAK signaling pathway in fibroblasts
|
---|---|
Published in |
Respiratory Research, September 2018
|
DOI | 10.1186/s12931-018-0863-3 |
Pubmed ID | |
Authors |
Gyong Hwa Hong, So-Young Park, Hyouk-Soo Kwon, Bo-Ram Bang, Jaechun Lee, Sang-Yeob Kim, Chan-Gi Pack, Soohyun Kim, Keun-Ai Moon, Tae-Bum Kim, Hee-Bom Moon, You Sook Cho |
Abstract |
Fibrosis in severe asthma often leads to irreversible organ dysfunction. However, the mechanism that regulates fibrosis remains poorly understood. Interleukin (IL)-32 plays a role in several chronic inflammatory diseases, including severe asthma. In this study, we investigated whether IL-32 is involved in fibrosis progression in the lungs. Murine models of chronic airway inflammation induced by ovalbumin and Aspergillus melleus protease and bleomycin-induced pulmonary fibrosis were employed. We evaluated the degree of tissue fibrosis after treatment with recombinant IL-32γ (rIL-32γ). Expression of fibronectin and α-smooth muscle actin (α-SMA) was examined and the transforming growth factor (TGF)-β-related signaling pathways was evaluated in activated human lung fibroblasts (MRC-5 cells) treated with rIL-32γ. rIL-32γ significantly attenuated collagen deposition and α-SMA production in both mouse models. rIL-32γ inhibited the production of fibronectin and α-SMA in MRC-5 cells stimulated with TGF-β. Additionally, rIL-32γ suppressed activation of the integrin-FAK-paxillin signaling axis but had no effect on the Smad and non-Smad signaling pathways. rIL-32γ localized outside of MRC-5 cells and inhibited the interaction between integrins and the extracellular matrix without directly binding to intracellular FAK and paxillin. These results demonstrate that IL-32γ has anti-fibrotic effects and is a novel target for preventing fibrosis. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 20 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 4 | 20% |
Unspecified | 3 | 15% |
Researcher | 3 | 15% |
Student > Bachelor | 2 | 10% |
Student > Ph. D. Student | 1 | 5% |
Other | 0 | 0% |
Unknown | 7 | 35% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 20% |
Unspecified | 3 | 15% |
Immunology and Microbiology | 3 | 15% |
Agricultural and Biological Sciences | 1 | 5% |
Psychology | 1 | 5% |
Other | 1 | 5% |
Unknown | 7 | 35% |