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Neem oil increases the efficiency of the entomopathogenic fungus Metarhizium anisopliae for the control of Aedes aegypti (Diptera: Culicidae) larvae

Overview of attention for article published in Parasites & Vectors, December 2015
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Title
Neem oil increases the efficiency of the entomopathogenic fungus Metarhizium anisopliae for the control of Aedes aegypti (Diptera: Culicidae) larvae
Published in
Parasites & Vectors, December 2015
DOI 10.1186/s13071-015-1280-9
Pubmed ID
Authors

Simone A. Gomes, Adriano R. Paula, Anderson Ribeiro, Catia O. P. Moraes, Jonathan W. A. B. Santos, Carlos P. Silva, Richard I. Samuels

Abstract

Entomopathogenic fungi are potential candidates for use in integrated vector management and many isolates are compatible with synthetic and natural insecticides. Neem oil was tested separately and in combination with the entomopathogenic fungus Metarhizium anisopliae against larvae of the dengue vector Aedes aegypti. Our aim was to increase the effectiveness of the fungus for the control of larval mosquito populations. Commercially available neem oil was used at concentrations ranging from 0.0001 to 1 %. Larval survival rates were monitored over a 7 day period following exposure to neem. The virulence of the fungus M. anisopliae was confirmed using five conidial concentrations (1 × 10(5) to 1 × 10(9) conidia mL(-1)) and survival monitored over 7 days. Two concentrations of fungal conidia were then tested together with neem (0.001 %). Survival curve comparisons were carried out using the Log-rank test and end-point survival rates were compared using one-way ANOVA. 1 % neem was toxic to A. aegypti larvae reducing survival to 18 % with S50 of 2 days. Neem had no effect on conidial germination or fungal vegetative growth in vitro. Larval survival rates were reduced to 24 % (S50 = 3 days) when using 1 × 10(9) conidia mL(-1). Using 1 × 10(8) conidia mL(-1), 30 % survival (S50 = 3 days) was observed. We tested a "sub-lethal" neem concentration (0.001 %) together with these concentrations of conidia. For combinations of neem + fungus, the survival rates were significantly lower than the survival rates seen for fungus alone or for neem alone. Using a combination of 1 × 10(7) conidia mL(-1) + neem (0.001 %), the survival rates were 36 %, whereas exposure to the fungus alone resulted in 74 % survival and exposure to neem alone resulted in 78 % survival. When using 1 × 10(8) conidia mL(-1), the survival curves were modified, with a combination of the fungus + neem resulting in 12 % survival, whilst the fungus alone at this concentration also significantly reduced survival rates (28 %). The use of adjuvants is an important strategy for maintaining/increasing fungal virulence and/or shelf-life. The addition of neem to conidial suspensions improved virulence, significantly reducing larval survival times and percentages.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 63 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 63 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 13 21%
Student > Master 8 13%
Researcher 8 13%
Student > Postgraduate 3 5%
Student > Ph. D. Student 3 5%
Other 7 11%
Unknown 21 33%
Readers by discipline Count As %
Agricultural and Biological Sciences 17 27%
Biochemistry, Genetics and Molecular Biology 9 14%
Immunology and Microbiology 4 6%
Environmental Science 2 3%
Pharmacology, Toxicology and Pharmaceutical Science 2 3%
Other 7 11%
Unknown 22 35%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 December 2015.
All research outputs
#15,352,477
of 22,836,570 outputs
Outputs from Parasites & Vectors
#3,385
of 5,467 outputs
Outputs of similar age
#230,601
of 393,178 outputs
Outputs of similar age from Parasites & Vectors
#97
of 150 outputs
Altmetric has tracked 22,836,570 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,467 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one is in the 30th percentile – i.e., 30% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 393,178 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 150 others from the same source and published within six weeks on either side of this one. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.