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Excision of staphylococcal cassette chromosome mec in methicillin-resistant Staphylococcus aureus assessed by quantitative PCR

Overview of attention for article published in BMC Research Notes, December 2015
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Title
Excision of staphylococcal cassette chromosome mec in methicillin-resistant Staphylococcus aureus assessed by quantitative PCR
Published in
BMC Research Notes, December 2015
DOI 10.1186/s13104-015-1815-3
Pubmed ID
Authors

Miloš Stojanov, P. Moreillon, O. Sakwinska

Abstract

Methicillin-resistance in staphylococci is conferred by the mecA gene, located on the genomic island Staphylococcal Cassette Chromosome mec (SCCmec). SCCmec mobility relies on the Ccr recombinases, which catalyze insertion and excision form the host's chromosome. Although being a crucial step in its horizontal transfer, little is known about the dynamics of SCCmec excision. A quantitative PCR-based method was used to measure the rate of SCCmec excision by amplifying the chromosome-chromosome junction and the circularized SCCmec resulting from excision. SCCmec excision rate was measured in methicillin-resistant Staphylococcus aureus (MRSA) strain N315 at various growth times in broth cultures. In the present experimental settings, excision of SCCmec occurred at a rate of approximately 2 × 10(-6) in MRSA N315. This work brings new insights in the poorly understood SCCmec excision process. The results presented herein suggest a model in which excision occurs during a limited period of time at the early stages of growth.

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The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Peru 1 5%
Unknown 19 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 25%
Researcher 4 20%
Student > Bachelor 4 20%
Student > Master 2 10%
Student > Doctoral Student 1 5%
Other 1 5%
Unknown 3 15%
Readers by discipline Count As %
Immunology and Microbiology 6 30%
Biochemistry, Genetics and Molecular Biology 5 25%
Agricultural and Biological Sciences 2 10%
Social Sciences 1 5%
Medicine and Dentistry 1 5%
Other 0 0%
Unknown 5 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 September 2016.
All research outputs
#19,627,490
of 24,138,997 outputs
Outputs from BMC Research Notes
#3,117
of 4,363 outputs
Outputs of similar age
#293,512
of 401,334 outputs
Outputs of similar age from BMC Research Notes
#109
of 146 outputs
Altmetric has tracked 24,138,997 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,363 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.9. This one is in the 16th percentile – i.e., 16% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 401,334 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 14th percentile – i.e., 14% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 146 others from the same source and published within six weeks on either side of this one. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.