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New insights into the fungal community from the raw genomic sequence data of fig wasp Ceratosolen solmsi

Overview of attention for article published in BMC Microbiology, February 2015
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Title
New insights into the fungal community from the raw genomic sequence data of fig wasp Ceratosolen solmsi
Published in
BMC Microbiology, February 2015
DOI 10.1186/s12866-015-0370-3
Pubmed ID
Authors

Li-Hua Niu, Xiu-Feng Song, Shun-Min He, Peng Zhang, Ning-Xin Wang, Yi Li, Da-Wei Huang

Abstract

To date, biologists have discovered a large amount of valuable information from assembled genomes, but the abundant microbial data that is hidden in the raw genomic sequence data of plants and animals is usually ignored. In this study, the richness and composition of fungal community were determined in the raw genomic sequence data of Ceratosolen solmsi (RGSD-CS). To avoid the interference from sequences of C. solmsi, the unmapped raw data (about 17.1%) was obtained by excluding the assembled genome of C. solmsi from RGSD-CS. Comparing two fungal reference datasets, internal transcribed spacer (ITS) and large ribosomal subunit (LSU) of rRNA, the ITS dataset discovered a more diverse fungal community and was therefore selected as the reference dataset for evaluating the fungal community based on the unmapped raw data. The threshold of 95% sequence identity revealed many more matched fungal reads and fungal richness in the unmapped raw data than those by identities above 95%. Based on the threshold of 95% sequence identity, the fungal community of RGSD-CS was primarily composed of Saccharomycetes (88.4%) and two other classes (Agaricomycetes and Sordariomycetes, 8.3% in total). Compared with the fungal community of other reported fig wasps, Agaricomycetes and Eurotiomycetes were found to be unique to C. solmsi. In addition, the ratio of total fungal reads to RGSD-CS was estimated to be at least 4.8 × 10(-3), which indicated that a large amount of fungal data was contained in RGSD-CS. However, rarefaction measure indicated that a deeper sequencing coverage with RGSD-CS was required to discover the entire fungal community of C. solmsi. This study investigated the richness and composition of fungal community in RGSD-CS and provided new insights into the efficient study of microbial diversity using raw genomic sequence data.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 26 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 10 38%
Student > Ph. D. Student 4 15%
Student > Master 3 12%
Student > Bachelor 2 8%
Student > Doctoral Student 2 8%
Other 4 15%
Unknown 1 4%
Readers by discipline Count As %
Agricultural and Biological Sciences 16 62%
Biochemistry, Genetics and Molecular Biology 4 15%
Environmental Science 2 8%
Medicine and Dentistry 1 4%
Unknown 3 12%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 January 2016.
All research outputs
#20,300,248
of 22,837,982 outputs
Outputs from BMC Microbiology
#2,691
of 3,192 outputs
Outputs of similar age
#301,456
of 357,982 outputs
Outputs of similar age from BMC Microbiology
#50
of 64 outputs
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