Title |
The natural compound Guttiferone F sensitizes prostate cancer to starvation induced apoptosis via calcium and JNK elevation
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Published in |
BMC Cancer, April 2015
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DOI | 10.1186/s12885-015-1292-z |
Pubmed ID | |
Authors |
Xin Li, Yuanzhi Lao, Hong Zhang, Xiaoyu Wang, Hongsheng Tan, Zhixiu Lin, Hongxi Xu |
Abstract |
In a cytotoxicity screen in serum-free medium, Guttiferone F showed strong growth inhibitory effect against prostate cancer cells. Prostate cancer cells LNCaP and PC3 were treated with Guttiferone F in serum depleted medium. Sub-G1 phase distributions were estimated with flow cytometry. Mitochondrial disruption was observed under confocal microscope using Mitotracker Red staining. Gene and protein expression changes were detected by real-time PCR and Western blotting. Ca(2+) elevation was examined by Fluo-4 staining under fluorescence microscope. PC3 xenografts in mice were examined by immunohistochemical analysis. Guttiferone F had strong growth inhibitory effect against prostate cancer cell lines under serum starvation. It induced a significant increase in sub-G1 fraction and DNA fragmentation. In serum-free medium, Guttiferone F triggered mitochondria dependent apoptosis by regulating Bcl-2 family proteins. In addition, Guttiferone F attenuated the androgen receptor expression and phosphorylation of ERK1/2, while activating the phosphorylation of JNK and Ca(2+) flux. Combination of caloric restriction with Guttiferone F in vivo could increase the antitumor effect without causing toxicity. Guttiferone F induced prostate cancer cell apoptosis under serum starvation via Ca(2+) elevation and JNK activation. Combined with caloric restriction, Guttiferone F exerted significant growth inhibition of PC3 cells xenograft in vivo. Guttiferone F is therefore a potential anti-cancer compound. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 33 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 7 | 21% |
Student > Ph. D. Student | 5 | 15% |
Student > Bachelor | 4 | 12% |
Researcher | 4 | 12% |
Student > Doctoral Student | 3 | 9% |
Other | 5 | 15% |
Unknown | 5 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Pharmacology, Toxicology and Pharmaceutical Science | 5 | 15% |
Biochemistry, Genetics and Molecular Biology | 5 | 15% |
Agricultural and Biological Sciences | 5 | 15% |
Medicine and Dentistry | 4 | 12% |
Nursing and Health Professions | 3 | 9% |
Other | 3 | 9% |
Unknown | 8 | 24% |