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Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA), Minas Gerais, Brazil

Overview of attention for article published in Parasites & Vectors, April 2015
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Title
Genetic homogeneity among Leishmania (Leishmania) infantum isolates from dog and human samples in Belo Horizonte Metropolitan Area (BHMA), Minas Gerais, Brazil
Published in
Parasites & Vectors, April 2015
DOI 10.1186/s13071-015-0837-y
Pubmed ID
Authors

Thais Almeida Marques da Silva, Luciana Inácia Gomes, Edward Oliveira, Wendel Coura-Vital, Letícia de Azevedo Silva, Fabiano Sviatopolk-Mirsky Pais, Henrique Gama Ker, Alexandre Barbosa Reis, Ana Rabello, Mariangela Carneiro

Abstract

Certain municipalities in the Belo Horizonte Metropolitan Area (BHMA), Minas Gerais, Brazil, have the highest human visceral leishmaniasis (VL) mortality rates in the country and also demonstrate high canine seropositivity. In Brazil, the etiologic agent of VL is Leishmania (Leishmania) infantum. The aim of this study was to evaluate the intraspecific genetic variability of parasites from humans and from dogs with different clinical forms of VL in five municipalities of BHMA using PCR-RFLP and two target genes: kinetoplast DNA (kDNA) and gp63. In total, 45 samples of DNA extracted from clinical samples (n = 35) or L. infantum culture (n = 10) were evaluated. These samples originated from three groups: adults (with or without Leishmania/HIV co-infection; n = 14), children (n = 18) and dogs (n = 13). The samples were amplified for the kDNA target using the MC1 and MC2 primers (447 bp), while the Sg1 and Sg2 (1330 bp) primers were used for the gp63 glycoprotein target gene. The restriction enzyme patterns of all the samples tested were monomorphic. These findings reveal a high degree of genetic homogeneity for the evaluated gene targets among L. infantum samples isolated from different hosts and representing different clinical forms of VL in the municipalities of BHMA studied.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 71 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 2 3%
Peru 1 1%
Unknown 68 96%

Demographic breakdown

Readers by professional status Count As %
Student > Master 12 17%
Student > Doctoral Student 8 11%
Researcher 7 10%
Student > Bachelor 7 10%
Student > Ph. D. Student 6 8%
Other 8 11%
Unknown 23 32%
Readers by discipline Count As %
Medicine and Dentistry 12 17%
Biochemistry, Genetics and Molecular Biology 10 14%
Immunology and Microbiology 5 7%
Agricultural and Biological Sciences 5 7%
Veterinary Science and Veterinary Medicine 4 6%
Other 9 13%
Unknown 26 37%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 January 2016.
All research outputs
#18,434,182
of 22,837,982 outputs
Outputs from Parasites & Vectors
#4,229
of 5,467 outputs
Outputs of similar age
#192,748
of 263,997 outputs
Outputs of similar age from Parasites & Vectors
#85
of 127 outputs
Altmetric has tracked 22,837,982 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,467 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one is in the 11th percentile – i.e., 11% of its peers scored the same or lower than it.
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We're also able to compare this research output to 127 others from the same source and published within six weeks on either side of this one. This one is in the 18th percentile – i.e., 18% of its contemporaries scored the same or lower than it.