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Molecular diagnosis and phylogenetic analysis of human papillomavirus type-16 from suspected patients in Pakistan

Overview of attention for article published in Infectious Agents and Cancer, January 2016
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  • Above-average Attention Score compared to outputs of the same age (51st percentile)
  • Good Attention Score compared to outputs of the same age and source (72nd percentile)

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42 Mendeley
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Title
Molecular diagnosis and phylogenetic analysis of human papillomavirus type-16 from suspected patients in Pakistan
Published in
Infectious Agents and Cancer, January 2016
DOI 10.1186/s13027-016-0047-z
Pubmed ID
Authors

Abdullah Abdullah, Muhammad Qasim, Muhammad Shafiq, Muhammad Ijaz, Shamsa Parveen, Shahnaz Murtaza, Qamar Javed, Salman Akbar Malik, Shahida Husain Tarar, Sajid Mehmood, Abdul Sami, Syed Muhammad Saqlan Naqvi, Muhammad Zeeshan Hyder

Abstract

Human Papillomavirus (HPV) is well known pathogen that can cause benign and malignant tumors in humans, yet there is very little information regarding HPV types prevalent in Pakistan. A total of 92 cervical secretions were collected from suspected married female patients and used for DNA isolation using a novel isolation method. The samples were tested through Polymerase Chain Reaction (PCR) using already reported primers MY09/MY11, GP5/GP6, GP5+/GP6+, CP65/CP70, CP66/CP69 and SPF1/SPF2 and with those developed in this study including HRT1 and HRT2 primer sets for typing HPV types and HACTB primer set for human beta actin gene as internal positive control. Sequencing and phylogenetic analyses were performed for two isolates to determine circulating HPV types. PCR with HRT1 and HRT2 indicated 2 (2.17 %) patients were positive for HPV type- 16 while 1 (1.08 %) with HPV type 18. Sequencing and phylogenetic analysis of isolates confirmed HPV type-16 in genus alpha 9 which have 99 % homology with already reported HPV from Japan and Costa Rica. This is the first report of HPV type-16 genus alpha 9 in Pakistan and the reported assay and sequence data will serve as valuable tools in further epidemiological studies for HPV surveillance to improve public health, especially of females in Pakistan.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 42 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 21%
Researcher 6 14%
Student > Bachelor 5 12%
Student > Master 5 12%
Student > Postgraduate 2 5%
Other 5 12%
Unknown 10 24%
Readers by discipline Count As %
Medicine and Dentistry 8 19%
Agricultural and Biological Sciences 6 14%
Biochemistry, Genetics and Molecular Biology 6 14%
Nursing and Health Professions 3 7%
Psychology 2 5%
Other 7 17%
Unknown 10 24%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 January 2016.
All research outputs
#13,685,294
of 23,613,071 outputs
Outputs from Infectious Agents and Cancer
#169
of 541 outputs
Outputs of similar age
#189,686
of 398,291 outputs
Outputs of similar age from Infectious Agents and Cancer
#4
of 11 outputs
Altmetric has tracked 23,613,071 research outputs across all sources so far. This one is in the 41st percentile – i.e., 41% of other outputs scored the same or lower than it.
So far Altmetric has tracked 541 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 7.3. This one has gotten more attention than average, scoring higher than 67% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 398,291 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.
We're also able to compare this research output to 11 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.