Title |
Development and usefulness of an immunochromatographic device to detect antibodies for rapid diagnosis of human gnathostomiasis
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Published in |
Parasites & Vectors, January 2016
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DOI | 10.1186/s13071-016-1294-y |
Pubmed ID | |
Authors |
Penchom Janwan, Pewpan M. Intapan, Hiroshi Yamasaki, Rutchanee Rodpai, Porntip Laummaunwai, Tongjit Thanchomnang, Oranuch Sanpool, Kaoru Kobayashi, Katsuyoshi Takayama, Yukuharu Kobayashi, Wanchai Maleewong |
Abstract |
Human gnathostomiasis is a serious tropical disease, which is often overlooked. There is an urgent need to improve tools to aid the potential diagnosis of the disease in endemic regions. To overcome this, we produced the immunochromatographic test (ICT) kit for a rapid and simple diagnosis of human gnathostomiasis. The recombinant protein (named rGslic18) was applied to ICT kit as the antigen. The diagnostic value of ICT kit was evaluated using serum samples from parasitologically proven and clinically suspected gnathostomiasis patients, healthy volunteers and patients with other parasitic diseases. The ICT kit exhibited quite high sensitivity (93.75 %) and specificity (97.01 %). The ICT kit is simple, convenient and easy to implement and expected to provide reliable diagnostic results for human gnathostomiasis. It also will be a promising diagnostic tool not only for large-scale epidemiological surveys in endemic or remote areas where diagnostic facilities are poor but also for a rapid clinical diagnosis in the bedside laboratory. |
X Demographics
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 50% |
Science communicators (journalists, bloggers, editors) | 1 | 50% |
Mendeley readers
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Unknown | 13 | 100% |
Demographic breakdown
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Student > Bachelor | 3 | 23% |
Researcher | 2 | 15% |
Lecturer > Senior Lecturer | 1 | 8% |
Lecturer | 1 | 8% |
Librarian | 1 | 8% |
Other | 2 | 15% |
Unknown | 3 | 23% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 3 | 23% |
Immunology and Microbiology | 2 | 15% |
Arts and Humanities | 1 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 8% |
Agricultural and Biological Sciences | 1 | 8% |
Other | 2 | 15% |
Unknown | 3 | 23% |