↓ Skip to main content

IL-4 orchestrates STAT6-mediated DNA demethylation leading to dendritic cell differentiation

Overview of attention for article published in Genome Biology (Online Edition), January 2016
Altmetric Badge

About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (90th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (52nd percentile)

Mentioned by

twitter
31 tweeters

Citations

dimensions_citation
82 Dimensions

Readers on

mendeley
117 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
IL-4 orchestrates STAT6-mediated DNA demethylation leading to dendritic cell differentiation
Published in
Genome Biology (Online Edition), January 2016
DOI 10.1186/s13059-015-0863-2
Pubmed ID
Authors

Roser Vento-Tormo, Carlos Company, Javier Rodríguez-Ubreva, Lorenzo de la Rica, José M. Urquiza, Biola M. Javierre, Radhakrishnan Sabarinathan, Ana Luque, Manel Esteller, Josep M. Aran, Damiana Álvarez-Errico, Esteban Ballestar

Abstract

The role of cytokines in establishing specific transcriptional programmes in innate immune cells has long been recognized. However, little is known about how these extracellular factors instruct innate immune cell epigenomes to engage specific differentiation states. Human monocytes differentiate under inflammatory conditions into effector cells with non-redundant functions, such as dendritic cells and macrophages. In this context, interleukin 4 (IL-4) and granulocyte macrophage colony-stimulating factor (GM-CSF) drive dendritic cell differentiation, whereas GM-CSF alone leads to macrophage differentiation. Here, we investigate the role of IL-4 in directing functionally relevant dendritic-cell-specific DNA methylation changes. A comparison of DNA methylome dynamics during differentiation from human monocytes to dendritic cells and macrophages identified gene sets undergoing dendritic-cell-specific or macrophage-specific demethylation. Demethylation is TET2-dependent and is essential for acquiring proper dendritic cell and macrophage identity. Most importantly, activation of the JAK3-STAT6 pathway, downstream of IL-4, is required for the acquisition of the dendritic-cell-specific demethylation and expression signature, following STAT6 binding. A constitutively activated form of STAT6 is able to bypass IL-4 upstream signalling and instruct dendritic-cell-specific functional DNA methylation changes. Our study is the first description of a cytokine-mediated sequence of events leading to direct gene-specific demethylation in innate immune cell differentiation.

Twitter Demographics

The data shown below were collected from the profiles of 31 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 117 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Japan 1 <1%
United States 1 <1%
Belgium 1 <1%
Unknown 114 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 32 27%
Researcher 28 24%
Student > Master 16 14%
Student > Bachelor 7 6%
Student > Doctoral Student 6 5%
Other 14 12%
Unknown 14 12%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 40 34%
Agricultural and Biological Sciences 29 25%
Immunology and Microbiology 20 17%
Medicine and Dentistry 5 4%
Business, Management and Accounting 1 <1%
Other 6 5%
Unknown 16 14%

Attention Score in Context

This research output has an Altmetric Attention Score of 16. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 February 2016.
All research outputs
#1,799,452
of 21,604,458 outputs
Outputs from Genome Biology (Online Edition)
#1,645
of 3,990 outputs
Outputs of similar age
#37,416
of 405,458 outputs
Outputs of similar age from Genome Biology (Online Edition)
#140
of 292 outputs
Altmetric has tracked 21,604,458 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 91st percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,990 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 27.6. This one has gotten more attention than average, scoring higher than 58% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 405,458 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 90% of its contemporaries.
We're also able to compare this research output to 292 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.