↓ Skip to main content

Biological impact of xeno-free chemically defined cryopreservation medium on amniotic epithelial cells

Overview of attention for article published in Stem Cell Research & Therapy, January 2016
Altmetric Badge

About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (83rd percentile)
  • Good Attention Score compared to outputs of the same age and source (71st percentile)

Mentioned by

blogs
1 blog
twitter
2 tweeters

Citations

dimensions_citation
17 Dimensions

Readers on

mendeley
46 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Biological impact of xeno-free chemically defined cryopreservation medium on amniotic epithelial cells
Published in
Stem Cell Research & Therapy, January 2016
DOI 10.1186/s13287-015-0258-z
Pubmed ID
Authors

Toshio Miki, Wisia Wong, Elton Zhou, Anthony Gonzalez, Irving Garcia, Brendan H. Grubbs

Abstract

Amnion-derived stem cells have been proposed for cell replacement therapy and tissue regeneration. An easily accessible cell source, the placenta, allows us to potentially establish a bio-bank of cells for immunotype matched clinical applications. Several xeno-free (XF) cryopreservation media are currently available for pluripotent stem cells, however, these media have not yet been evaluated for the cryopreservation of amnion-derived stem cells. Human amniotic epithelial cells were collected using standard protocols, and stored at -160 °C in one of five commercially available media. Cells frozen in standard media containing fetal bovine serum served as controls. Cells were then thawed, and evaluated for viability, mitochondrial membrane stability, and senescence status. Quantitative real time PCR was utilized to assess for expression of stem cell genes, and flow cytometry was used to identify the stem cell surface markers. Cell recovery and repopulation assays indicated no significant difference between XF media versus standard cryopreservation medium. In addition, no impact was observed on the senescence status, the cytostructural or mitochondrial morphology between the tested cryopreservation media. Differences were observed on the expression of stem cell marker genes (OCT4, SOX2, and NANOG) and a cell surface marker (TRA1-60) following cryopreservation in different chemically defined XF media, however, these were not statistically significant. Xeno-free cryopreservation of human amnion-derived stem cells is feasible and can be standardized to establish a bio-bank with human amnion-derived stem cells for future clinical application. Optimization of this media may allow for improved preservation of stem cell-like characteristics.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 46 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 1 2%
Unknown 45 98%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 10 22%
Researcher 10 22%
Professor > Associate Professor 5 11%
Student > Ph. D. Student 5 11%
Student > Doctoral Student 3 7%
Other 9 20%
Unknown 4 9%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 20%
Medicine and Dentistry 8 17%
Agricultural and Biological Sciences 8 17%
Engineering 5 11%
Immunology and Microbiology 3 7%
Other 5 11%
Unknown 8 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 January 2016.
All research outputs
#878,789
of 6,982,524 outputs
Outputs from Stem Cell Research & Therapy
#104
of 535 outputs
Outputs of similar age
#50,061
of 305,462 outputs
Outputs of similar age from Stem Cell Research & Therapy
#12
of 42 outputs
Altmetric has tracked 6,982,524 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 535 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one has done well, scoring higher than 80% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 305,462 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 83% of its contemporaries.
We're also able to compare this research output to 42 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.