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The evaluation and application of multilocus variable number tandem repeat analysis (MLVA) for the molecular epidemiological study of Salmonella enterica subsp. enterica serovar Enteritidis infection

Overview of attention for article published in Annals of Clinical Microbiology and Antimicrobials, January 2016
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Title
The evaluation and application of multilocus variable number tandem repeat analysis (MLVA) for the molecular epidemiological study of Salmonella enterica subsp. enterica serovar Enteritidis infection
Published in
Annals of Clinical Microbiology and Antimicrobials, January 2016
DOI 10.1186/s12941-016-0119-3
Pubmed ID
Authors

Yao Liu, Xiaolu Shi, Yinghui Li, Qiongcheng Chen, Min Jiang, Wanli Li, Yaqun Qiu, Yiman Lin, Yixiang Jiang, Biao Kan, Qun Sun, Qinghua Hu

Abstract

Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) is one of the most prevalent Salmonella serotypes that cause gastroenteritis worldwide and the most prevalent serotype causing Salmonella infections in China. A rapid molecular typing method with high throughput and good epidemiological discrimination is urgently needed for detecting the outbreaks and finding the source for effective control of S. Enteritidis infections. In this study, 194 strains which included 47 from six outbreaks that were well-characterized epidemiologically were analyzed with pulse field gel electrophoresis (PFGE) and multilocus variable number tandem repeat analysis (MLVA). Seven VNTR loci published by the US Center for Disease Control and Prevention (CDC) were used to evaluate and develop MLVA scheme for S. Enteritidis molecular subtyping by comparing with PFGE, and then MLVA was applied to the suspected outbreaks detection. All S. Enteritidis isolates were analyzed with MLVA to establish a MLVA database in Shenzhen, Guangdong province, China to facilitate the detection of S. Enteritidis infection clusters. There were 33 MLVA types and 29 PFGE patterns among 147 sporadic isolates. These two measures had Simpson indices of 0.7701 and 0.8043, respectively, which did not differ significantly. Epidemiological concordance was evaluated by typing 47 isolates from six epidemiologically well-characterized outbreaks and it did not differ for PFGE and MLVA. We applied the well established MLVA method to detect two S. Enteritidis foodborne outbreaks and find their sources successfully in 2014. A MLVA database of 491 S. Enteritidis strains isolated from 2004 to 2014 was established for the surveillance of clusters in the future. MLVA typing of S. Enteritidis would be an effective tool for early warning and epidemiological surveillance of S. Enteritidis infections.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 31 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 9 29%
Student > Master 4 13%
Student > Doctoral Student 3 10%
Student > Ph. D. Student 3 10%
Student > Postgraduate 2 6%
Other 4 13%
Unknown 6 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 19%
Biochemistry, Genetics and Molecular Biology 5 16%
Medicine and Dentistry 3 10%
Veterinary Science and Veterinary Medicine 2 6%
Immunology and Microbiology 2 6%
Other 5 16%
Unknown 8 26%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 February 2016.
All research outputs
#17,783,561
of 22,842,950 outputs
Outputs from Annals of Clinical Microbiology and Antimicrobials
#396
of 608 outputs
Outputs of similar age
#269,858
of 396,349 outputs
Outputs of similar age from Annals of Clinical Microbiology and Antimicrobials
#26
of 29 outputs
Altmetric has tracked 22,842,950 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 608 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 396,349 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 29 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.