Title |
Huh-7 cell line as an alternative cultural model for the production of human like erythropoietin (EPO)
|
---|---|
Published in |
Journal of Translational Medicine, November 2011
|
DOI | 10.1186/1479-5876-9-186 |
Pubmed ID | |
Authors |
Humera Kausar, Sana Gull, Bushra Ijaz, Waqar Ahmad, Muhammad Tahir Sarwar, Zafar Iqbal, Zafar Nawaz, Sheikh Riazuddin, Sajida Hassan |
Abstract |
Erythropoietin (EPO) is a glycoprotein hormone which is required to regulate the production of red blood cells. Deficiency of EPO is known to cause anemia in chronically infected renal patients and they require regular blood transfusion. Availability of recombinant EPO has eliminated the need for blood transfusion and now it is extensively used for the treatment of anemia. Glycosylation of erythropoietin is essential for its secretion, stability, protein conformation and biological activity. However, maintenance of human like glycosylation pattern during manufacturing of EPO is a major challenge in biotechnology. Currently, Chinese hamster ovary (CHO) cell line is used for the commercial production of erythropoietin but this cell line does not maintain glycosylation resembling human system. With the trend to eliminate non-human constituent from biopharmaceutical products, as a preliminary approach, we have investigated the potential of human hepatoma cell line (Huh-7) to produce recombinant EPO. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 49 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 10 | 20% |
Researcher | 6 | 12% |
Student > Doctoral Student | 4 | 8% |
Student > Master | 4 | 8% |
Other | 2 | 4% |
Other | 6 | 12% |
Unknown | 17 | 35% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 10 | 20% |
Biochemistry, Genetics and Molecular Biology | 7 | 14% |
Medicine and Dentistry | 4 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 6% |
Chemical Engineering | 2 | 4% |
Other | 3 | 6% |
Unknown | 20 | 41% |