Title |
A pilot study for channel catfish whole genome sequencing and de novo assembly
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Published in |
BMC Genomics, December 2011
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DOI | 10.1186/1471-2164-12-629 |
Pubmed ID | |
Authors |
Yanliang Jiang, Jianguo Lu, Eric Peatman, Huseyin Kucuktas, Shikai Liu, Shaolin Wang, Fanyue Sun, Zhanjiang Liu |
Abstract |
Recent advances in next-generation sequencing technologies have drastically increased throughput and significantly reduced sequencing costs. However, the average read lengths in next-generation sequencing technologies are short as compared with that of traditional Sanger sequencing. The short sequence reads pose great challenges for de novo sequence assembly. As a pilot project for whole genome sequencing of the catfish genome, here we attempt to determine the proper sequence coverage, the proper software for assembly, and various parameters used for the assembly of a BAC physical map contig spanning approximately a million of base pairs. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 5 | 6% |
France | 2 | 2% |
Norway | 1 | 1% |
Germany | 1 | 1% |
Sweden | 1 | 1% |
Brazil | 1 | 1% |
Unknown | 72 | 87% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 24 | 29% |
Researcher | 22 | 27% |
Student > Master | 8 | 10% |
Other | 6 | 7% |
Professor > Associate Professor | 4 | 5% |
Other | 12 | 14% |
Unknown | 7 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 52 | 63% |
Biochemistry, Genetics and Molecular Biology | 9 | 11% |
Computer Science | 5 | 6% |
Medicine and Dentistry | 3 | 4% |
Environmental Science | 2 | 2% |
Other | 2 | 2% |
Unknown | 10 | 12% |