Title |
MTA1 promotes the invasion and migration of non-small cell lung cancer cells by downregulating miR-125b
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Published in |
Journal of Experimental & Clinical Cancer Research, May 2013
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DOI | 10.1186/1756-9966-32-33 |
Pubmed ID | |
Authors |
Yiyi Li, Yilan Chao, Yuan Fang, Jian Wang, Min Wang, Hong Zhang, Min Ying, Xiaoxia Zhu, Haofei Wang |
Abstract |
The metastasis-associated gene 1 (MTA1) has been identified as one critical regulator of tumor metastasis. Previously, we identified miR-125b as a downregualted miRNA in non-small cell lung cancer (NSCLC) cell line upon MTA1 depletion. However, the role of miR-125b and MTA1 in the regulation of NSCLC metastasis remains unclear. Stable MTA1 knockdown NSCLC cell lines 95D and SPC-A-1 were established by transfection with MTA1 shRNA. The effects of MTA1 depletion on the expression of miR-125b and cell migration and invasion were examined by real-time PCR, wound healing and matrigel invasion assay. MTA1 knockdown led to the upregulation of miR-125b level in NSCLC cells. Furthermore, MTA1 knockdown reduced while miR-125b inhibitor enhanced cell migration and invasion of NSCLC cells. Notably, miR-125b inhibitor antagonized MTA1 siRNA induced inhibition of cell migration and invasion. MTA1 and miR-125b have antagonistic effects on the migration and invasion of NSCLC cells. The newly identified MTA1-miR-125b axis will help further elucidate the molecular mechanism of NSCLC progression and suggest that ectopic expression of miR-125b is a potentially new therapeutic regimen against NSCLC metastasis. |
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Librarian | 1 | 6% |
Other | 0 | 0% |
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Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
Other | 0 | 0% |
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