Title |
High throughput generation of promoter reporter (GFP) transgenic lines of low expressing genes in Arabidopsis and analysis of their expression patterns
|
---|---|
Published in |
Plant Methods, August 2010
|
DOI | 10.1186/1746-4811-6-18 |
Pubmed ID | |
Authors |
Yong-Li Xiao, Julia C Redman, Erin L Monaghan, Jun Zhuang, Beverly A Underwood, William A Moskal, Wei Wang, Hank C Wu, Christopher D Town |
Abstract |
Although the complete genome sequence and annotation of Arabidopsis were released at the end of year 2000, it is still a great challenge to understand the function of each gene in the Arabidopsis genome. One way to understand the function of genes on a genome-wide scale is expression profiling by microarrays. However, the expression level of many genes in Arabidopsis genome cannot be detected by microarray experiments. In addition, there are many more novel genes that have been discovered by experiments or predicted by new gene prediction programs. Another way to understand the function of individual genes is to investigate their in vivo expression patterns by reporter constructs in transgenic plants which can provide basic information on the patterns of gene expression. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Portugal | 1 | <1% |
Austria | 1 | <1% |
Australia | 1 | <1% |
Brazil | 1 | <1% |
Canada | 1 | <1% |
Argentina | 1 | <1% |
Spain | 1 | <1% |
United States | 1 | <1% |
Unknown | 126 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 44 | 33% |
Researcher | 34 | 25% |
Student > Master | 13 | 10% |
Student > Doctoral Student | 11 | 8% |
Student > Postgraduate | 9 | 7% |
Other | 15 | 11% |
Unknown | 8 | 6% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 109 | 81% |
Biochemistry, Genetics and Molecular Biology | 7 | 5% |
Chemistry | 3 | 2% |
Business, Management and Accounting | 1 | <1% |
Medicine and Dentistry | 1 | <1% |
Other | 1 | <1% |
Unknown | 12 | 9% |