@niemasd @pvanheus Yes of course it is 😉 https://t.co/QDZgQePN1K
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@BortzGroup @BrianRWasik @Virus_Evolution On benchmarking, one of my very favorite intrahost studies comes from @NathanGrubaugh et al. in @GenomeBiology (you may already be familiar). They also describe other approaches that may be of interest with Nanopor
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An amplicon-based sequencing framework for accurately measuring intrahost virus diversity using PrimalSeq and iVar - PubMed https://t.co/NmttCredpA
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9/ Within the same critique, the authors question why a 10x min coverage threshold was chosen, implying that a lower threshold (5x) is more appropriate. 10x (or more) is standard in viral genomics, e.g. the iVar Consensus default is 10 https://t.co/etEFSaB