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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Revisiting chromatin binding of the Arabidopsis UV-B photoreceptor UVR8
|
|---|---|
| Published in |
BMC Plant Biology, February 2016
|
| DOI | 10.1186/s12870-016-0732-5 |
| Pubmed ID | |
| Authors |
Melanie Binkert, Carlos D. Crocco, Babatunde Ekundayo, Kelvin Lau, Sarah Raffelberg, Kimberley Tilbrook, Ruohe Yin, Richard Chappuis, Thomas Schalch, Roman Ulm |
| Abstract |
Plants perceive UV-B through the UV RESISTANCE LOCUS 8 (UVR8) photoreceptor and UVR8 activation leads to changes in gene expression such as those associated with UV-B acclimation and stress tolerance. Albeit functionally unrelated, UVR8 shows some homology with RCC1 (Regulator of Chromatin Condensation 1) proteins from non-plant organisms at the sequence level. These proteins act as guanine nucleotide exchange factors for Ran GTPases and bind chromatin via histones. Subsequent to the revelation of this sequence homology, evidence was presented showing that UVR8 activity involves interaction with chromatin at the loci of some target genes through histone binding. This suggested a UVR8 mode-of-action intimately and directly linked with gene transcription. However, several aspects of UVR8 chromatin association remained undefined, namely the impact of UV-B on the process and how UVR8 chromatin association related to the transcription factor ELONGATED HYPOCOTYL 5 (HY5), which is important for UV-B signalling and has overlapping chromatin targets. Therefore, we have investigated UVR8 chromatin association in further detail. Unlike the claims of previous studies, our chromatin immunoprecipitation (ChIP) experiments do not confirm UVR8 chromatin association. In contrast to human RCC1, recombinant UVR8 also does not bind nucleosomes in vitro. Moreover, fusion of a VP16 activation domain to UVR8 did not alter expression of proposed UVR8 target genes in transient gene expression assays. Finally, comparison of the Drosophila DmRCC1 and the Arabidopsis UVR8 crystal structures revealed that critical histone- and DNA-interaction residues apparent in DmRCC1 are not conserved in UVR8. This has led us to conclude that the cellular activity of UVR8 likely does not involve its specific binding to chromatin at target genes. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 63 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 2% |
| New Zealand | 1 | 2% |
| Netherlands | 1 | 2% |
| Austria | 1 | 2% |
| Unknown | 59 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 17 | 27% |
| Student > Ph. D. Student | 13 | 21% |
| Student > Bachelor | 8 | 13% |
| Student > Master | 6 | 10% |
| Professor | 5 | 8% |
| Other | 11 | 17% |
| Unknown | 3 | 5% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 30 | 48% |
| Biochemistry, Genetics and Molecular Biology | 15 | 24% |
| Chemistry | 3 | 5% |
| Physics and Astronomy | 2 | 3% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
| Other | 2 | 3% |
| Unknown | 10 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 March 2016.
All research outputs
#14,431,072
of 23,577,654 outputs
Outputs from BMC Plant Biology
#1,096
of 3,320 outputs
Outputs of similar age
#207,854
of 403,738 outputs
Outputs of similar age from BMC Plant Biology
#23
of 67 outputs
Altmetric has tracked 23,577,654 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,320 research outputs from this source. They receive a mean Attention Score of 3.0. This one has gotten more attention than average, scoring higher than 64% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 403,738 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 67 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 61% of its contemporaries.