Title |
The new anti-actin agent dihydrohalichondramide reveals fenestrae-forming centers in hepatic endothelial cells
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Published in |
BMC Molecular and Cell Biology, March 2002
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DOI | 10.1186/1471-2121-3-7 |
Pubmed ID | |
Authors |
Filip Braet, Ilan Spector, Nava Shochet, Phillip Crews, Tatsuo Higa, Eline Menu, Ronald de Zanger, Eddie Wisse |
Abstract |
Liver sinusoidal endothelial cells (LSECs) react to different anti-actin agents by increasing their number of fenestrae. A new structure related to fenestrae formation could be observed when LSECs were treated with misakinolide. In this study, we investigated the effects of two new actin-binding agents on fenestrae dynamics. High-resolution microscopy, including immunocytochemistry and a combination of fluorescence- and scanning electron microscopy was applied. Halichondramide and dihydrohalichondramide disrupt microfilaments within 10 minutes and double the number of fenestrae in 30 minutes. Dihydrohalichondramide induces fenestrae-forming centers, whereas halichondramide only revealed fenestrae-forming centers without attached rows of fenestrae with increasing diameter. Correlative microscopy showed the absence of actin filaments (F-actin) in sieve plates and fenestrae-forming centers. Comparable experiments on umbilical vein endothelial cells and bone marrow sinusoidal endothelial cells revealed cell contraction without the appearance of fenestrae or fenestrae-forming centers. (I) A comparison of all anti-actin agents tested so far, revealed that the only activity that misakinolide and dihydrohalichondramide have in common is their barbed end capping activity; (II) this activity seems to slow down the process of fenestrae formation to such extent that it becomes possible to resolve fenestrae-forming centers; (III) fenestrae formation resulting from microfilament disruption is probably unique to LSECs. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 28 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 13 | 46% |
Student > Ph. D. Student | 4 | 14% |
Student > Master | 3 | 11% |
Professor | 2 | 7% |
Student > Bachelor | 1 | 4% |
Other | 1 | 4% |
Unknown | 4 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 14 | 50% |
Biochemistry, Genetics and Molecular Biology | 6 | 21% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
Immunology and Microbiology | 1 | 4% |
Physics and Astronomy | 1 | 4% |
Other | 1 | 4% |
Unknown | 4 | 14% |