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Antibiotics inhibit sphere-forming ability in suspension culture

Overview of attention for article published in Cancer Cell International, February 2016
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2 tweeters

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Readers on

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34 Mendeley
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Title
Antibiotics inhibit sphere-forming ability in suspension culture
Published in
Cancer Cell International, February 2016
DOI 10.1186/s12935-016-0277-6
Pubmed ID
Authors

Sébastien Relier, Laura Yazdani, Oualid Ayad, Armelle Choquet, Jean-François Bourgaux, Michel Prudhomme, Julie Pannequin, Françoise Macari, Alexandre David

Abstract

This last decade, a lot of emphasis has been placed on developing new cancer cell culture models, closer to in vivo condition, in order to test new drugs and therapies. In the case of colorectal cancer, the use of patient biopsies to seed 3D primary cultures and mimic tumor initiation necessitates the use of antibiotics to prevent microbial intestinal contamination. However, not only long term use of antibiotics may mask the presence of low levels of microbial contamination, it may also impact cancer cell phenotype. In this study we tested the impact of penicillin-streptomycin cocktail addition in both monolayer and suspension culture. To ensure the reliability of our observations we used six different cell lines and each experiment was performed in triplicate. Results were analyzed with Student's t test. We show that penicillin-streptomycin cocktail inhibits the sphere-forming ability of six cancer cell lines in suspension culture though it has no impact in monolayer culture. We correlate this effect with a significant decrease of cancer stem cells pool which holds self-renewal potential. Overall, this study warns against systematic addition of antibiotics in growth medium and raises the interesting possibility of using antibiotics to target cancer stem cells.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 34 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 7 21%
Researcher 6 18%
Student > Master 4 12%
Student > Ph. D. Student 4 12%
Lecturer 2 6%
Other 4 12%
Unknown 7 21%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 12 35%
Agricultural and Biological Sciences 6 18%
Medicine and Dentistry 2 6%
Veterinary Science and Veterinary Medicine 1 3%
Nursing and Health Professions 1 3%
Other 3 9%
Unknown 9 26%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 February 2016.
All research outputs
#16,942,509
of 21,005,902 outputs
Outputs from Cancer Cell International
#816
of 1,541 outputs
Outputs of similar age
#269,393
of 375,840 outputs
Outputs of similar age from Cancer Cell International
#1
of 1 outputs
Altmetric has tracked 21,005,902 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,541 research outputs from this source. They receive a mean Attention Score of 2.8. This one is in the 30th percentile – i.e., 30% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 375,840 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them