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Mendeley readers
| Title |
A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila
|
|---|---|
| Published in |
Genome Biology, September 2007
|
| DOI | 10.1186/gb-2007-8-9-r203 |
| Pubmed ID | |
| Authors |
Ramanuj DasGupta, Kent Nybakken, Matthew Booker, Bernard Mathey-Prevot, Foster Gonsalves, Binita Changkakoty, Norbert Perrimon |
| Abstract |
Off-target effects have been demonstrated to be a major source of false-positives in RNA interference (RNAi) high-throughput screens. In this study, we re-assess the previously published transcriptional reporter-based whole-genome RNAi screens for the Wingless and Hedgehog signaling pathways using second generation double-stranded RNA libraries. Furthermore, we investigate other factors that may influence the outcome of such screens, including cell-type specificity, robustness of reporters, and assay normalization, which determine the efficacy of RNAi-knockdown of target genes. |
Mendeley readers
The data shown below were compiled from readership statistics for 46 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 2% |
| China | 1 | 2% |
| Germany | 1 | 2% |
| Canada | 1 | 2% |
| Unknown | 42 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 12 | 26% |
| Student > Ph. D. Student | 9 | 20% |
| Professor | 7 | 15% |
| Professor > Associate Professor | 6 | 13% |
| Student > Bachelor | 3 | 7% |
| Other | 7 | 15% |
| Unknown | 2 | 4% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 23 | 50% |
| Biochemistry, Genetics and Molecular Biology | 16 | 35% |
| Nursing and Health Professions | 1 | 2% |
| Chemical Engineering | 1 | 2% |
| Computer Science | 1 | 2% |
| Other | 1 | 2% |
| Unknown | 3 | 7% |