Title |
Whole Genome Profiling provides a robust framework for physical mapping and sequencing in the highly complex and repetitive wheat genome
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Published in |
BMC Genomics, January 2012
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DOI | 10.1186/1471-2164-13-47 |
Pubmed ID | |
Authors |
Romain Philippe, Frédéric Choulet, Etienne Paux, Jan van Oeveren, Jifeng Tang, Alexander HJ Wittenberg, Antoine Janssen, Michiel JT van Eijk, Keith Stormo, Adriana Alberti, Patrick Wincker, Eduard Akhunov, Edwin van der Vossen, Catherine Feuillet |
Abstract |
Sequencing projects using a clone-by-clone approach require the availability of a robust physical map. The SNaPshot technology, based on pair-wise comparisons of restriction fragments sizes, has been used recently to build the first physical map of a wheat chromosome and to complete the maize physical map. However, restriction fragments sizes shared randomly between two non-overlapping BACs often lead to chimerical contigs and mis-assembled BACs in such large and repetitive genomes. Whole Genome Profiling (WGP™) was developed recently as a new sequence-based physical mapping technology and has the potential to limit this problem. |
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Geographical breakdown
Country | Count | As % |
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Argentina | 1 | 14% |
Spain | 1 | 14% |
Belgium | 1 | 14% |
United Kingdom | 1 | 14% |
Germany | 1 | 14% |
Unknown | 2 | 29% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 6 | 86% |
Scientists | 1 | 14% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Canada | 2 | 3% |
Italy | 1 | 1% |
Australia | 1 | 1% |
France | 1 | 1% |
Brazil | 1 | 1% |
Argentina | 1 | 1% |
Thailand | 1 | 1% |
Japan | 1 | 1% |
Unknown | 58 | 87% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 24 | 36% |
Student > Ph. D. Student | 12 | 18% |
Other | 7 | 10% |
Student > Master | 6 | 9% |
Student > Postgraduate | 5 | 7% |
Other | 12 | 18% |
Unknown | 1 | 1% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 50 | 75% |
Computer Science | 5 | 7% |
Biochemistry, Genetics and Molecular Biology | 4 | 6% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 1% |
Business, Management and Accounting | 1 | 1% |
Other | 3 | 4% |
Unknown | 3 | 4% |