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Successful expression of the Bordetella petrii nitrile hydratase activator P14K and the unnecessary role of Ser115

Overview of attention for article published in BMC Biotechnology, February 2016
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2 tweeters

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6 Dimensions

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Title
Successful expression of the Bordetella petrii nitrile hydratase activator P14K and the unnecessary role of Ser115
Published in
BMC Biotechnology, February 2016
DOI 10.1186/s12896-016-0252-2
Pubmed ID
Authors

Weifeng Sun, Longbao Zhu, Xianggui Chen, Ping Chen, Lingling Yang, Wenwu Ding, Zhemin Zhou, Yi Liu

Abstract

The activator P14K is necessary for the activation of nitrile hydratase (NHase). However, it is hard to be expressed heterogeneously. Although an N-terminal strep tagged P14K could be successfully expressed from Pseudomonas putida, various strategies for the over-expression of P14K are needed to facilitate further application of NHase. P14K was successfully expressed through fusing a his tag (his-P14K), and was over-expressed through fusing a gst tag (gst-P14K) at its N-terminus in the NHase of Bordetella petrii DSM 12804. The stability of gst-P14K was demonstrated to be higher than that of the his-P14K. In addition, the Ser115 in the characteristic motif CXLC-Ser115-C of the active center of NHase was found to be unnecessary for NHase maturation. Our results are not only useful for the NHase activator expression and the understanding of the role of Ser115 during NHase activation, but also helpful for other proteins with difficulty in heterologous expression.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 1 20%
Student > Doctoral Student 1 20%
Student > Master 1 20%
Unknown 2 40%
Readers by discipline Count As %
Immunology and Microbiology 1 20%
Chemistry 1 20%
Engineering 1 20%
Unknown 2 40%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 February 2016.
All research outputs
#10,951,560
of 14,414,062 outputs
Outputs from BMC Biotechnology
#568
of 769 outputs
Outputs of similar age
#170,545
of 267,602 outputs
Outputs of similar age from BMC Biotechnology
#1
of 1 outputs
Altmetric has tracked 14,414,062 research outputs across all sources so far. This one is in the 20th percentile – i.e., 20% of other outputs scored the same or lower than it.
So far Altmetric has tracked 769 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one is in the 22nd percentile – i.e., 22% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 267,602 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 30th percentile – i.e., 30% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them