Title |
Molecular detection of Mycobacterium bovis in cattle herds of the state of Pernambuco, Brazil
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Published in |
BMC Veterinary Research, February 2016
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DOI | 10.1186/s12917-016-0656-1 |
Pubmed ID | |
Authors |
Renata Duarte da Silva Cezar, Norma Lucena-Silva, Antônio Fernando Barbosa Batista Filho, Jonas de Melo Borges, Pollyane Raysa Fernandes de Oliveira, Érica Chaves Lúcio, Maíra Arruda-Lima, Vania Lucia de Assis Santana, José Wilton Pinheiro Junior |
Abstract |
The present study aimed to direct detect Mycobacterium bovis in milk (n = 401) and blood (n = 401) samples collected from 401 dairy cows of 20 properties located in the state of Pernambuco, Brazil, by real-time quantitative PCR (qPCR) targeting the region of difference 4 (RD4). Risk factors possibly associated with bovine tuberculosis (BTB) were also evaluated. Of the 802 samples analyzed, one milk (0.25 %) and eight blood (2 %) samples were positive for M. bovis in the qPCR and their identities were confirmed by sequencing. Animals positive for M. bovis were found in six (30 %) of the 20 properties visited. None of the risk factors evaluated were statistically associated with BTB. M. bovis DNA was detected in one milk sample what may pose a risk to public health because raw milk is commonly consumed in Brazil. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Brazil | 1 | 1% |
Unknown | 67 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 13 | 19% |
Student > Postgraduate | 10 | 15% |
Student > Bachelor | 6 | 9% |
Researcher | 6 | 9% |
Student > Ph. D. Student | 6 | 9% |
Other | 12 | 18% |
Unknown | 15 | 22% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 15 | 22% |
Veterinary Science and Veterinary Medicine | 13 | 19% |
Biochemistry, Genetics and Molecular Biology | 7 | 10% |
Nursing and Health Professions | 3 | 4% |
Immunology and Microbiology | 3 | 4% |
Other | 10 | 15% |
Unknown | 17 | 25% |