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The standard operating procedure of the DOE-JGI Metagenome Annotation Pipeline (MAP v.4)

Overview of attention for article published in Environmental Microbiome, February 2016
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Title
The standard operating procedure of the DOE-JGI Metagenome Annotation Pipeline (MAP v.4)
Published in
Environmental Microbiome, February 2016
DOI 10.1186/s40793-016-0138-x
Pubmed ID
Authors

Marcel Huntemann, Natalia N. Ivanova, Konstantinos Mavromatis, H. James Tripp, David Paez-Espino, Kristin Tennessen, Krishnaveni Palaniappan, Ernest Szeto, Manoj Pillay, I-Min A. Chen, Amrita Pati, Torben Nielsen, Victor M. Markowitz, Nikos C. Kyrpides

Abstract

The DOE-JGI Metagenome Annotation Pipeline (MAP v.4) performs structural and functional annotation for metagenomic sequences that are submitted to the Integrated Microbial Genomes with Microbiomes (IMG/M) system for comparative analysis. The pipeline runs on nucleotide sequences provided via the IMG submission site. Users must first define their analysis projects in GOLD and then submit the associated sequence datasets consisting of scaffolds/contigs with optional coverage information and/or unassembled reads in fasta and fastq file formats. The MAP processing consists of feature prediction including identification of protein-coding genes, non-coding RNAs and regulatory RNAs, as well as CRISPR elements. Structural annotation is followed by functional annotation including assignment of protein product names and connection to various protein family databases.

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Mendeley readers

The data shown below were compiled from readership statistics for 187 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 1 <1%
United States 1 <1%
France 1 <1%
Australia 1 <1%
Unknown 183 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 45 24%
Student > Ph. D. Student 41 22%
Student > Master 22 12%
Student > Bachelor 14 7%
Student > Doctoral Student 9 5%
Other 21 11%
Unknown 35 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 59 32%
Biochemistry, Genetics and Molecular Biology 36 19%
Environmental Science 20 11%
Immunology and Microbiology 10 5%
Computer Science 4 2%
Other 14 7%
Unknown 44 24%