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Karyotypic assignment of Sri Lankan Anopheles culicifacies species B and E does not correlate with cytochrome oxidase subunit I and microsatellite genotypes

Overview of attention for article published in Parasites & Vectors, June 2015
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Title
Karyotypic assignment of Sri Lankan Anopheles culicifacies species B and E does not correlate with cytochrome oxidase subunit I and microsatellite genotypes
Published in
Parasites & Vectors, June 2015
DOI 10.1186/s13071-015-0944-9
Pubmed ID
Authors

Sinnathamby N. Surendran, Nathan Truelove, Devojit K. Sarma, Pavilupillai J. Jude, Ranjan Ramasamy, Kanapathy Gajapathy, Lalanthika B. S. Peiris, S. H. P. Parakrama Karunaratne, Catherine Walton

Abstract

The identification of species B and E in the Anopheles culicifacies complex in the Indian subcontinent has been based on Y-chromosome karyotype. Since no detectable variations were previously found in DNA markers commonly used for sibling species identification, further molecular characterization using cytochrome oxidase subunit I (COI) and microsatellite markers was carried out on Y-chromosome karyotyped Anopheles culicifacies specie B and E from Unnichchai, Kallady and Ranawarunawa in Sri Lanka . COI sequence analysis (n = 22) revealed the presence of nine unique haplotypes with six in each species. Three haplotypes were shared by both species. The two sibling species had a pairwise FST value of 1.338 (p < 0.05) with the number of migrants (Nm) value <1. The genetic structure analysis resulted in two genetic clusters not 100 % associated with karyotypes. While none of the species B were incorrectly assigned two were inconclusive. Five out of 26 specimens karyotyped as species E were incorrectly assigned, while further 9 were inconclusive. The new molecular data support the existence of two genetically different populations of the Culicifacies Complex in Sri Lanka that are not associated with the Y-chromosome karyotype. Detailed analysis with more microsatellite markers and assortative mating experiments are needed to establish the presence of the two genetically distinct populations and relate them to Y-chromosome morphology.

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Geographical breakdown

Country Count As %
Unknown 28 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 4 14%
Student > Ph. D. Student 3 11%
Researcher 3 11%
Student > Master 3 11%
Other 2 7%
Other 4 14%
Unknown 9 32%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 21%
Biochemistry, Genetics and Molecular Biology 3 11%
Nursing and Health Professions 2 7%
Medicine and Dentistry 2 7%
Mathematics 1 4%
Other 4 14%
Unknown 10 36%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 March 2016.
All research outputs
#20,656,161
of 25,373,627 outputs
Outputs from Parasites & Vectors
#4,692
of 5,987 outputs
Outputs of similar age
#203,834
of 278,536 outputs
Outputs of similar age from Parasites & Vectors
#89
of 122 outputs
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