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Mendeley readers
| Title |
Bioluminescence imaging of leukemia cell lines in vitro and in mouse xenografts: effects of monoclonal and polyclonal cell populations on intensity and kinetics of photon emission
|
|---|---|
| Published in |
Journal of Hematology & Oncology, January 2013
|
| DOI | 10.1186/1756-8722-6-10 |
| Pubmed ID | |
| Authors |
Sandra Christoph, Jennifer Schlegel, Francesca Alvarez-Calderon, Yong-Mi Kim, Luis N Brandao, Deborah DeRyckere, Douglas K Graham |
| Abstract |
We investigated the utility of bioluminescence imaging (BLI) using firefly luciferase in monoclonal and polyclonal populations of leukemia cells in vitro and in vivo. Monoclonal and polyclonal human lymphoid and myeloid leukemia cell lines transduced with firefly luciferase were used for BLI. Kinetics and dynamics of bioluminescence signal were cell line dependent. Luciferase expression decreased significantly over time in polyclonal leukemia cells in vitro. Transplantation of polyclonal luciferase-tagged cells in mice resulted in inconsistent signal intensity. After selection of monoclonal cell populations, luciferase activity was stable, equal kinetic and dynamic of bioluminescence intensity and strong correlation between cell number and light emission in vitro were observed. We obtained an equal development of leukemia burden detected by luciferase activity in NOD-scid-gamma mice after transplantation of monoclonal populations. The use of monoclonal leukemia cells selected for stable and equal luciferase activity is recommended for experiments in vitro and xenograft mouse models. The findings are highly significant for bioluminescence imaging focused on pre-clinical drug development. |
Mendeley readers
The data shown below were compiled from readership statistics for 67 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 1% |
| Unknown | 66 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 15 | 22% |
| Researcher | 12 | 18% |
| Student > Master | 11 | 16% |
| Student > Bachelor | 6 | 9% |
| Other | 4 | 6% |
| Other | 7 | 10% |
| Unknown | 12 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 21 | 31% |
| Agricultural and Biological Sciences | 14 | 21% |
| Medicine and Dentistry | 3 | 4% |
| Immunology and Microbiology | 3 | 4% |
| Veterinary Science and Veterinary Medicine | 2 | 3% |
| Other | 11 | 16% |
| Unknown | 13 | 19% |