Title |
Rapid alterations of cell cycle control proteins in human T lymphocytes in microgravity
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Published in |
Cell Communication and Signaling, January 2012
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DOI | 10.1186/1478-811x-10-1 |
Pubmed ID | |
Authors |
Cora S Thiel, Katrin Paulsen, Gesine Bradacs, Karolin Lust, Svantje Tauber, Claudia Dumrese, Andre Hilliger, Kathrin Schoppmann, Josefine Biskup, Nadine Gölz, Chen Sang, Urs Ziegler, Karl-Heinrich Grote, Frauke Zipp, Fengyuan Zhuang, Frank Engelmann, Ruth Hemmersbach, Augusto Cogoli, Oliver Ullrich |
Abstract |
In our study we aimed to identify rapidly reacting gravity-responsive mechanisms in mammalian cells in order to understand if and how altered gravity is translated into a cellular response. In a combination of experiments using "functional weightlessness" provided by 2D-clinostats and real microgravity provided by several parabolic flight campaigns and compared to in-flight-1g-controls, we identified rapid gravity-responsive reactions inside the cell cycle regulatory machinery of human T lymphocytes. In response to 2D clinorotation, we detected an enhanced expression of p21 Waf1/Cip1 protein within minutes, less cdc25C protein expression and enhanced Ser147-phosphorylation of cyclinB1 after CD3/CD28 stimulation. Additionally, during 2D clinorotation, Tyr-15-phosphorylation occurred later and was shorter than in the 1 g controls. In CD3/CD28-stimulated primary human T cells, mRNA expression of the cell cycle arrest protein p21 increased 4.1-fold after 20s real microgravity in primary CD4+ T cells and 2.9-fold in Jurkat T cells, compared to 1 g in-flight controls after CD3/CD28 stimulation. The histone acetyltransferase (HAT) inhibitor curcumin was able to abrogate microgravity-induced p21 mRNA expression, whereas expression was enhanced by a histone deacetylase (HDAC) inhibitor. Therefore, we suppose that cell cycle progression in human T lymphocytes requires Earth gravity and that the disturbed expression of cell cycle regulatory proteins could contribute to the breakdown of the human immune system in space. |
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