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Mendeley readers
Attention Score in Context
| Title |
Design of a VLP-nanovehicle for CYP450 enzymatic activity delivery
|
|---|---|
| Published in |
Journal of Nanobiotechnology, October 2015
|
| DOI | 10.1186/s12951-015-0127-z |
| Pubmed ID | |
| Authors |
Lorena Sánchez-Sánchez, Alejandro Tapia-Moreno, Karla Juarez-Moreno, Dustin P. Patterson, Ruben D. Cadena-Nava, Trevor Douglas, Rafael Vazquez-Duhalt |
| Abstract |
The intracellular delivery of enzymes for therapeutic use has a promising future for the treatment of several diseases such as genetic disorders and cancer. Virus-like particles offer an interesting platform for enzymatic delivery to targeted cells because of their great cargo capacity and the enhancement of the biocatalyst stability towards several factors important in the practical application of these nanoparticles. We have designed a nano-bioreactor based on the encapsulation of a cytochrome P450 (CYP) inside the capsid derived from the bacteriophage P22. An enhanced peroxigenase, CYPBM3, was selected as a model enzyme because of its potential in enzyme prodrug therapy. A total of 109 enzymes per capsid were encapsulated with a 70 % retention of activity for cytochromes with the correct incorporation of the heme cofactor. Upon encapsulation, the stability of the enzyme towards protease degradation and acidic pH was increased. Cytochrome P450 activity was delivered into Human cervix carcinoma cells via transfecting P22-CYP nanoparticles with lipofectamine. This work provides a clear demonstration of the potential of biocatalytic virus-like particles as medical relevant enzymatic delivery vehicles for clinical applications. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 99 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Mexico | 1 | 1% |
| United States | 1 | 1% |
| Netherlands | 1 | 1% |
| Unknown | 96 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 25 | 25% |
| Student > Master | 12 | 12% |
| Researcher | 8 | 8% |
| Student > Bachelor | 8 | 8% |
| Student > Doctoral Student | 5 | 5% |
| Other | 10 | 10% |
| Unknown | 31 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 20 | 20% |
| Agricultural and Biological Sciences | 13 | 13% |
| Chemistry | 12 | 12% |
| Engineering | 4 | 4% |
| Materials Science | 4 | 4% |
| Other | 9 | 9% |
| Unknown | 37 | 37% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 March 2016.
All research outputs
#18,447,592
of 22,856,968 outputs
Outputs from Journal of Nanobiotechnology
#897
of 1,420 outputs
Outputs of similar age
#200,559
of 278,788 outputs
Outputs of similar age from Journal of Nanobiotechnology
#12
of 17 outputs
Altmetric has tracked 22,856,968 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,420 research outputs from this source. They receive a mean Attention Score of 3.5. This one is in the 13th percentile – i.e., 13% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 278,788 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 17 others from the same source and published within six weeks on either side of this one. This one is in the 23rd percentile – i.e., 23% of its contemporaries scored the same or lower than it.