Title |
Development of an ELISA-array for simultaneous detection of five encephalitis viruses
|
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Published in |
Virology Journal, February 2012
|
DOI | 10.1186/1743-422x-9-56 |
Pubmed ID | |
Authors |
Xiaoping Kang, Yuchang Li, Li Fan, Fang Lin, Jingjing Wei, Xiaolei Zhu, Yi Hu, Jing Li, Guohui Chang, Qingyu Zhu, Hong Liu, Yinhui Yang |
Abstract |
Japanese encephalitis virus(JEV), tick-borne encephalitis virus(TBEV), and eastern equine encephalitis virus (EEEV) can cause symptoms of encephalitis. Establishment of accurate and easy methods by which to detect these viruses is essential for the prevention and treatment of associated infectious diseases. Currently, there are still no multiple antigen detection methods available clinically. An ELISA-array, which detects multiple antigens, is easy to handle, and inexpensive, has enormous potential in pathogen detection. An ELISA-array method for the simultaneous detection of five encephalitis viruses was developed in this study. Seven monoclonal antibodies against five encephalitis-associated viruses were prepared and used for development of the ELISA-array. The ELISA-array assay is based on a "sandwich" ELISA format and consists of viral antibodies printed directly on 96-well microtiter plates, allowing for direct detection of 5 viruses. The developed ELISA-array proved to have similar specificity and higher sensitivity compared with the conventional ELISAs. This method was validated by different viral cultures and three chicken eggs inoculated with infected patient serum. The results demonstrated that the developed ELISA-array is sensitive and easy to use, which would have potential for clinical use. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 1 | 2% |
Portugal | 1 | 2% |
Unknown | 39 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 10 | 24% |
Student > Master | 10 | 24% |
Student > Bachelor | 6 | 15% |
Student > Ph. D. Student | 6 | 15% |
Other | 2 | 5% |
Other | 3 | 7% |
Unknown | 4 | 10% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 4 | 10% |
Immunology and Microbiology | 4 | 10% |
Veterinary Science and Veterinary Medicine | 4 | 10% |
Other | 6 | 15% |
Unknown | 4 | 10% |