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Enhancing functional production of a chaperone-dependent lipase in Escherichia coli using the dual expression cassette plasmid

Overview of attention for article published in Microbial Cell Factories, March 2012
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  • Good Attention Score compared to outputs of the same age (67th percentile)
  • Average Attention Score compared to outputs of the same age and source

Mentioned by

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1 X user
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1 patent

Citations

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20 Dimensions

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44 Mendeley
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Title
Enhancing functional production of a chaperone-dependent lipase in Escherichia coli using the dual expression cassette plasmid
Published in
Microbial Cell Factories, March 2012
DOI 10.1186/1475-2859-11-29
Pubmed ID
Authors

ThiDinh Quyen, ChiHai Vu, GiangThi Thu Le

Abstract

The lipase subfamilies I.1 and I.2 show more than 33% homology in the amino acid sequences and most members share another common property that their genes are clustered with the secondary genes whose protein products are required for folding the lipase into an active conformation and secretion into the culture medium. In previous studies, the lipase (LipA) and its chaperone (LipB) from Ralstonia sp. M1 were overexpressed in E. coli and the lipase was successfully refolded in vitro. The purpose of this study was to enhance the production of the active lipase LipA from Ralstonia sp. M1 in the heterologous host E. coli without in vitro refolding process, using two-plasmid co-expression systems and dual expression cassette plasmid systems.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Malaysia 1 2%
Unknown 43 98%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 8 18%
Student > Ph. D. Student 8 18%
Student > Master 8 18%
Student > Doctoral Student 6 14%
Student > Postgraduate 4 9%
Other 4 9%
Unknown 6 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 20 45%
Biochemistry, Genetics and Molecular Biology 10 23%
Chemistry 4 9%
Engineering 3 7%
Computer Science 1 2%
Other 1 2%
Unknown 5 11%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 October 2013.
All research outputs
#7,959,659
of 25,373,627 outputs
Outputs from Microbial Cell Factories
#547
of 1,823 outputs
Outputs of similar age
#50,861
of 168,144 outputs
Outputs of similar age from Microbial Cell Factories
#8
of 18 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 1,823 research outputs from this source. They receive a mean Attention Score of 4.9. This one has gotten more attention than average, scoring higher than 69% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 168,144 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.
We're also able to compare this research output to 18 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.