Title |
The JCVI standard operating procedure for annotating prokaryotic metagenomic shotgun sequencing data
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Published in |
Environmental Microbiome, April 2010
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DOI | 10.4056/sigs.651139 |
Pubmed ID | |
Authors |
David M. Tanenbaum, Johannes Goll, Sean Murphy, Prateek Kumar, Nikhat Zafar, Mathangi Thiagarajan, Ramana Madupu, Tanja Davidsen, Leonid Kagan, Saul Kravitz, Douglas B. Rusch, Shibu Yooseph |
Abstract |
The JCVI metagenomics analysis pipeline provides for the efficient and consistent annotation of shotgun metagenomics sequencing data for sampling communities of prokaryotic organisms. The process can be equally applied to individual sequence reads from traditional Sanger capillary electrophoresis sequences, newer technologies such as 454 pyrosequencing, or sequence assemblies derived from one or more of these data types. It includes the analysis of both coding and non-coding genes, whether full-length or, as is often the case for shotgun metagenomics, fragmentary. The system is designed to provide the best-supported conservative functional annotation based on a combination of trusted homology-based scientific evidence and computational assertions and an annotation value hierarchy established through extensive manual curation. The functional annotation attributes assigned by this system include gene name, gene symbol, GO terms, EC numbers, and JCVI functional role categories. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 5 | 3% |
Brazil | 5 | 3% |
United States | 4 | 3% |
Denmark | 2 | 1% |
Spain | 2 | 1% |
Argentina | 2 | 1% |
Sweden | 1 | <1% |
India | 1 | <1% |
Italy | 1 | <1% |
Other | 4 | 3% |
Unknown | 120 | 82% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 45 | 31% |
Student > Ph. D. Student | 34 | 23% |
Student > Master | 13 | 9% |
Student > Bachelor | 12 | 8% |
Student > Doctoral Student | 11 | 7% |
Other | 23 | 16% |
Unknown | 9 | 6% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 97 | 66% |
Biochemistry, Genetics and Molecular Biology | 15 | 10% |
Computer Science | 5 | 3% |
Environmental Science | 4 | 3% |
Engineering | 3 | 2% |
Other | 8 | 5% |
Unknown | 15 | 10% |