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Detection of Wolbachia in field-collected Aedes aegypti mosquitoes in metropolitan Manila, Philippines

Overview of attention for article published in Parasites & Vectors, July 2019
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Title
Detection of Wolbachia in field-collected Aedes aegypti mosquitoes in metropolitan Manila, Philippines
Published in
Parasites & Vectors, July 2019
DOI 10.1186/s13071-019-3629-y
Pubmed ID
Authors

Thaddeus M. Carvajal, Kazuki Hashimoto, Reza Kurniawan Harnandika, Divina M Amalin, Kozo Watanabe

Abstract

Recent reports reveal the presence of Wolbachia in Ae. aegypti. Our study presents additional support for Wolbachia infection in Ae. aegypti by screening field-collected adult mosquitoes using two Wolbachia-specific molecular makers. A total of 672 Ae. aegypti adult mosquitoes were collected from May 2014 to January 2015 in Metropolitan Manila. Each individual sample was processed and screened for the presence of Wolbachia by selected markers, Wolbachia-specific 16S rDNA and its surface protein (wsp), under optimized PCR conditions and sequenced. Totals of 113 (16.8%) and 89 (13.2%) individual mosquito samples were determined to be infected with Wolbachia using the wsp and 16S rDNA markers, respectively. The Ae. aegpyti wsp sample sequences were similar or identical to five known Wolbachia strains belonging to supergroups A and B while the majority of 16S rDNA sample sequences were similar to strains belonging to supergroup B. Overall, 80 (11.90%) individual mosquito samples showed positive amplifications in both markers and 69% showed congruence in supergroup identification (supergroup B). By utilizing two Wolbachia-specific molecular makers, our study demonstrated the presence of Wolbachia from individual Ae. aegypti samples. Our results showed a low Wolbachia infection rate and inferred the detected strains belong to either supergroups A and B.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 114 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 114 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 17 15%
Student > Ph. D. Student 14 12%
Researcher 11 10%
Student > Bachelor 9 8%
Student > Postgraduate 5 4%
Other 21 18%
Unknown 37 32%
Readers by discipline Count As %
Agricultural and Biological Sciences 30 26%
Biochemistry, Genetics and Molecular Biology 20 18%
Medicine and Dentistry 6 5%
Immunology and Microbiology 5 4%
Nursing and Health Professions 2 2%
Other 13 11%
Unknown 38 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 July 2019.
All research outputs
#18,838,150
of 23,343,453 outputs
Outputs from Parasites & Vectors
#4,314
of 5,558 outputs
Outputs of similar age
#259,075
of 347,116 outputs
Outputs of similar age from Parasites & Vectors
#98
of 133 outputs
Altmetric has tracked 23,343,453 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,558 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one is in the 11th percentile – i.e., 11% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 347,116 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 14th percentile – i.e., 14% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 133 others from the same source and published within six weeks on either side of this one. This one is in the 17th percentile – i.e., 17% of its contemporaries scored the same or lower than it.