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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Quantitative MNase-seq accurately maps nucleosome occupancy levels
|
|---|---|
| Published in |
Genome Biology, September 2019
|
| DOI | 10.1186/s13059-019-1815-z |
| Pubmed ID | |
| Authors |
Răzvan V. Chereji, Terri D. Bryson, Steven Henikoff |
| Abstract |
Micrococcal nuclease (MNase) is widely used to map nucleosomes. However, its aggressive endo-/exo-nuclease activities make MNase-seq unreliable for determining nucleosome occupancies, because cleavages within linker regions produce oligo- and mono-nucleosomes, whereas cleavages within nucleosomes destroy them. Here, we introduce a theoretical framework for predicting nucleosome occupancies and an experimental protocol with appropriate spike-in normalization that confirms our theory and provides accurate occupancy levels over an MNase digestion time course. As with human cells, we observe no overall differences in nucleosome occupancies between Drosophila euchromatin and heterochromatin, which implies that heterochromatic compaction does not reduce MNase accessibility of linker DNA. |
X Demographics
The data shown below were collected from the profiles of 23 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 9 | 39% |
| United Kingdom | 3 | 13% |
| Australia | 1 | 4% |
| France | 1 | 4% |
| India | 1 | 4% |
| Switzerland | 1 | 4% |
| Canada | 1 | 4% |
| Unknown | 6 | 26% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 11 | 48% |
| Members of the public | 10 | 43% |
| Practitioners (doctors, other healthcare professionals) | 1 | 4% |
| Science communicators (journalists, bloggers, editors) | 1 | 4% |
Mendeley readers
The data shown below were compiled from readership statistics for 197 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 197 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 37 | 19% |
| Student > Ph. D. Student | 36 | 18% |
| Student > Bachelor | 20 | 10% |
| Student > Master | 18 | 9% |
| Student > Doctoral Student | 12 | 6% |
| Other | 22 | 11% |
| Unknown | 52 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 85 | 43% |
| Agricultural and Biological Sciences | 34 | 17% |
| Computer Science | 4 | 2% |
| Immunology and Microbiology | 4 | 2% |
| Medicine and Dentistry | 4 | 2% |
| Other | 12 | 6% |
| Unknown | 54 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 43. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 February 2024.
All research outputs
#958,236
of 25,385,509 outputs
Outputs from Genome Biology
#679
of 4,470 outputs
Outputs of similar age
#20,228
of 351,033 outputs
Outputs of similar age from Genome Biology
#19
of 72 outputs
Altmetric has tracked 25,385,509 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,470 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 27.6. This one has done well, scoring higher than 84% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 351,033 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 94% of its contemporaries.
We're also able to compare this research output to 72 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.