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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Orthogonal inactivation of influenza and the creation of detergent resistant viral aggregates: towards a novel vaccine strategy
|
|---|---|
| Published in |
Virology Journal, March 2012
|
| DOI | 10.1186/1743-422x-9-72 |
| Pubmed ID | |
| Authors |
Julie M Belanger, Yossef Raviv, Mathias Viard, Ulrich Baxa, Robert Blumenthal |
| Abstract |
It has been previously shown that enveloped viruses can be inactivated using aryl azides, such as 1-iodo-5-azidonaphthalene (INA), plus UVA irradiation with preservation of surface epitopes in the inactivated virus preparations. Prolonged UVA irradiation in the presence of INA results in ROS-species formation, which in turn results in detergent resistant viral protein fractions. Herein, we characterize the applicability of this technique to inactivate influenza. It is shown that influenza virus + INA (100 micromolar) + UVA irradiation for 30 minutes results in a significant (p < 0.05) increase in pelletablehemagglutinin after Triton X-100 treatment followed by ultracentrifugation. Additionally, characterization of the virus suspension by immunogold labeling in cryo-EM, and viral pellet characterization via immunoprecipitation with a neutralizing antibody, shows preservation of neutralization epitopes after this treatment. These orthogonally inactivated viral preparations with detergent resistant fractions are being explored as a novel route for safe, effective inactivated vaccines generated from a variety of enveloped viruses. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 1 | 4% |
| Unknown | 24 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 9 | 36% |
| Student > Ph. D. Student | 5 | 20% |
| Lecturer | 2 | 8% |
| Student > Master | 2 | 8% |
| Lecturer > Senior Lecturer | 1 | 4% |
| Other | 3 | 12% |
| Unknown | 3 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 36% |
| Immunology and Microbiology | 4 | 16% |
| Medicine and Dentistry | 3 | 12% |
| Biochemistry, Genetics and Molecular Biology | 2 | 8% |
| Psychology | 1 | 4% |
| Other | 3 | 12% |
| Unknown | 3 | 12% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 March 2012.
All research outputs
#15,242,707
of 22,663,969 outputs
Outputs from Virology Journal
#1,934
of 3,028 outputs
Outputs of similar age
#102,163
of 160,299 outputs
Outputs of similar age from Virology Journal
#21
of 47 outputs
Altmetric has tracked 22,663,969 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,028 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 25.5. This one is in the 26th percentile – i.e., 26% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 160,299 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 47 others from the same source and published within six weeks on either side of this one. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.