Title |
Combination of nanoparticle-based therapeutic vaccination and transient ablation of regulatory T cells enhances anti-viral immunity during chronic retroviral infection
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Published in |
Retrovirology, April 2016
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DOI | 10.1186/s12977-016-0258-9 |
Pubmed ID | |
Authors |
Torben Knuschke, Olga Rotan, Wibke Bayer, Viktoriya Sokolova, Wiebke Hansen, Tim Sparwasser, Ulf Dittmer, Matthias Epple, Jan Buer, Astrid M. Westendorf |
Abstract |
Regulatory T cells (Tregs) have been shown to limit anti-viral immunity during chronic retroviral infection and to restrict vaccine-induced T cell responses. The objective of the study was to assess whether a combinational therapy of nanoparticle-based therapeutic vaccination and concomitant transient ablation of Tregs augments anti-viral immunity and improves virus control in chronically retrovirus-infected mice. Therefore, chronically Friend retrovirus (FV)-infected mice were immunized with calcium phosphate (CaP) nanoparticles functionalized with TLR9 ligand CpG and CD8(+) or CD4(+) T cell epitope peptides (GagL85-93 or Env gp70123-141) of FV. In addition, Tregs were ablated during the immunization process. Reactivation of CD4(+) and CD8(+) effector T cells was analysed and the viral loads were determined. Therapeutic vaccination of chronically FV-infected mice with functionalized CaP nanoparticles transiently reactivated cytotoxic CD8(+) T cells and significantly reduced the viral loads. Transient ablation of Tregs during nanoparticle-based therapeutic vaccination strongly enhanced anti-viral immunity and further decreased viral burden. Our data illustrate a crucial role for CD4(+) Foxp3(+) Tregs in the suppression of anti-viral T cell responses during therapeutic vaccination against chronic retroviral infection. Thus, the combination of transient Treg ablation and therapeutic nanoparticle-based vaccination confers robust and sustained anti-viral immunity. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Kenya | 1 | 33% |
United Kingdom | 1 | 33% |
Unknown | 1 | 33% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 67% |
Science communicators (journalists, bloggers, editors) | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 36 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 7 | 19% |
Researcher | 6 | 17% |
Student > Bachelor | 4 | 11% |
Student > Doctoral Student | 3 | 8% |
Student > Master | 3 | 8% |
Other | 5 | 14% |
Unknown | 8 | 22% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 7 | 19% |
Immunology and Microbiology | 4 | 11% |
Chemistry | 4 | 11% |
Medicine and Dentistry | 4 | 11% |
Biochemistry, Genetics and Molecular Biology | 3 | 8% |
Other | 7 | 19% |
Unknown | 7 | 19% |