Title |
Tailored enrichment strategy detects low abundant small noncoding RNAs in HIV-1 infected cells
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Published in |
Retrovirology, March 2012
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DOI | 10.1186/1742-4690-9-27 |
Pubmed ID | |
Authors |
Claudia F Althaus, Valentina Vongrad, Barbara Niederöst, Beda Joos, Francesca Di Giallonardo, Philip Rieder, Jovan Pavlovic, Alexandra Trkola, Huldrych F Günthard, Karin J Metzner, Marek Fischer |
Abstract |
The various classes of small noncoding RNAs (sncRNAs) are important regulators of gene expression across divergent types of organisms. While a rapidly increasing number of sncRNAs has been identified over recent years, the isolation of sncRNAs of low abundance remains challenging. Virally encoded sncRNAs, particularly those of RNA viruses, can be expressed at very low levels. This is best illustrated by HIV-1 where virus encoded sncRNAs represent approximately 0.1-1.0% of all sncRNAs in HIV-1 infected cells or were found to be undetected. Thus, we applied a novel, sequence targeted enrichment strategy to capture HIV-1 derived sncRNAs in HIV-1 infected primary CD4+ T-lymphocytes and macrophages that allows a greater than 100-fold enrichment of low abundant sncRNAs. |
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Unknown | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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South Africa | 1 | 2% |
Switzerland | 1 | 2% |
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Researcher | 10 | 24% |
Professor > Associate Professor | 4 | 10% |
Professor | 3 | 7% |
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Other | 6 | 14% |
Unknown | 7 | 17% |
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Biochemistry, Genetics and Molecular Biology | 5 | 12% |
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Chemistry | 1 | 2% |
Other | 0 | 0% |
Unknown | 9 | 21% |