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X Demographics
Mendeley readers
Attention Score in Context
| Title |
A simple method for plasma total vitamin C analysis suitable for routine clinical laboratory use
|
|---|---|
| Published in |
Nutrition Journal, April 2016
|
| DOI | 10.1186/s12937-016-0158-9 |
| Pubmed ID | |
| Authors |
Line Robitaille, L. John Hoffer |
| Abstract |
In-hospital hypovitaminosis C is highly prevalent but almost completely unrecognized. Medical awareness of this potentially important disorder is hindered by the inability of most hospital laboratories to determine plasma vitamin C concentrations. The availability of a simple, reliable method for analyzing plasma vitamin C could increase opportunities for routine plasma vitamin C analysis in clinical medicine. Plasma vitamin C can be analyzed by high performance liquid chromatography (HPLC) with electrochemical (EC) or ultraviolet (UV) light detection. We modified existing UV-HPLC methods for plasma total vitamin C analysis (the sum of ascorbic and dehydroascorbic acid) to develop a simple, constant-low-pH sample reduction procedure followed by isocratic reverse-phase HPLC separation using a purely aqueous low-pH non-buffered mobile phase. Although EC-HPLC is widely recommended over UV-HPLC for plasma total vitamin C analysis, the two methods have never been directly compared. We formally compared the simplified UV-HPLC method with EC-HPLC in 80 consecutive clinical samples. The simplified UV-HPLC method was less expensive, easier to set up, required fewer reagents and no pH adjustments, and demonstrated greater sample stability than many existing methods for plasma vitamin C analysis. When compared with the gold-standard EC-HPLC method in 80 consecutive clinical samples exhibiting a wide range of plasma vitamin C concentrations, it performed equivalently. The easy set up, simplicity and sensitivity of the plasma vitamin C analysis method described here could make it practical in a normally equipped hospital laboratory. Unlike any prior UV-HPLC method for plasma total vitamin C analysis, it was rigorously compared with the gold-standard EC-HPLC method and performed equivalently. Adoption of this method could increase the availability of plasma vitamin C analysis in clinical medicine. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 25% |
| Costa Rica | 1 | 25% |
| Germany | 1 | 25% |
| Unknown | 1 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Practitioners (doctors, other healthcare professionals) | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 135 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | <1% |
| Unknown | 134 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 19 | 14% |
| Student > Master | 15 | 11% |
| Student > Ph. D. Student | 14 | 10% |
| Student > Bachelor | 11 | 8% |
| Student > Postgraduate | 5 | 4% |
| Other | 22 | 16% |
| Unknown | 49 | 36% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 17 | 13% |
| Biochemistry, Genetics and Molecular Biology | 16 | 12% |
| Chemistry | 14 | 10% |
| Agricultural and Biological Sciences | 13 | 10% |
| Immunology and Microbiology | 6 | 4% |
| Other | 15 | 11% |
| Unknown | 54 | 40% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 June 2020.
All research outputs
#12,892,884
of 22,865,319 outputs
Outputs from Nutrition Journal
#973
of 1,431 outputs
Outputs of similar age
#136,736
of 299,499 outputs
Outputs of similar age from Nutrition Journal
#18
of 31 outputs
Altmetric has tracked 22,865,319 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,431 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 36.2. This one is in the 31st percentile – i.e., 31% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 299,499 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.
We're also able to compare this research output to 31 others from the same source and published within six weeks on either side of this one. This one is in the 41st percentile – i.e., 41% of its contemporaries scored the same or lower than it.