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Mendeley readers
Attention Score in Context
| Title |
A seeding based cellular assay of tauopathy
|
|---|---|
| Published in |
Molecular Neurodegeneration, April 2016
|
| DOI | 10.1186/s13024-016-0100-9 |
| Pubmed ID | |
| Authors |
Yin Xu, Heidi Martini-Stoica, Hui Zheng |
| Abstract |
Tauopathy is characterized by neurofibrillary tangles composed of insoluble hyperphosphorylated tau protein. Currently, cellular models that mimic neurofibrillary tangles in vitro are lacking. Previous studies indicate that neurofibrillary tangles form via a prion replication mechanism. In the present work, we establish a seeding based cellular model according to the prion hypothesis. We show that cellular soluble tau can be converted to insoluble tau by seeds from the brain lysate of rTg4510 mice or synthetically generated preformed tau fibrils (PFFs). The cellular insoluble tau exhibits classic features of neurofibrillary tangles. Using genetic and pharmacological methods, we demonstrate that inhibition of autophagy increases whereas enhancement of autophagy reduces insoluble tau in our seeding based cellular model. The insoluble tau can be detected and quantified by thioflavin-S staining, thus allowing us to adapt our cellular model to a high-content image-based screening platform. Our seeding based cellular model reproduces neurofibrillary tangle pathology in vitro and serves as a useful tool for studying tauopathy and identifying tau modulators. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 59 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 59 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 18 | 31% |
| Researcher | 12 | 20% |
| Student > Bachelor | 9 | 15% |
| Student > Doctoral Student | 4 | 7% |
| Professor | 2 | 3% |
| Other | 6 | 10% |
| Unknown | 8 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 16 | 27% |
| Biochemistry, Genetics and Molecular Biology | 14 | 24% |
| Neuroscience | 11 | 19% |
| Medicine and Dentistry | 4 | 7% |
| Business, Management and Accounting | 1 | 2% |
| Other | 3 | 5% |
| Unknown | 10 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 July 2018.
All research outputs
#3,129,922
of 22,865,319 outputs
Outputs from Molecular Neurodegeneration
#446
of 850 outputs
Outputs of similar age
#52,186
of 298,924 outputs
Outputs of similar age from Molecular Neurodegeneration
#12
of 18 outputs
Altmetric has tracked 22,865,319 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 850 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 14.2. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 298,924 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 82% of its contemporaries.
We're also able to compare this research output to 18 others from the same source and published within six weeks on either side of this one. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.