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CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq

Overview of attention for article published in Genome Biology, April 2016
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  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (95th percentile)
  • High Attention Score compared to outputs of the same age and source (87th percentile)

Citations

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939 Dimensions

Readers on

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1181 Mendeley
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3 CiteULike
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Title
CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq
Published in
Genome Biology, April 2016
DOI 10.1186/s13059-016-0938-8
Pubmed ID
Authors

Tamar Hashimshony, Naftalie Senderovich, Gal Avital, Agnes Klochendler, Yaron de Leeuw, Leon Anavy, Dave Gennert, Shuqiang Li, Kenneth J. Livak, Orit Rozenblatt-Rosen, Yuval Dor, Aviv Regev, Itai Yanai

Abstract

Single-cell transcriptomics requires a method that is sensitive, accurate, and reproducible. Here, we present CEL-Seq2, a modified version of our CEL-Seq method, with threefold higher sensitivity, lower costs, and less hands-on time. We implemented CEL-Seq2 on Fluidigm's C1 system, providing its first single-cell, on-chip barcoding method, and we detected gene expression changes accompanying the progression through the cell cycle in mouse fibroblast cells. We also compare with Smart-Seq to demonstrate CEL-Seq2's increased sensitivity relative to other available methods. Collectively, the improvements make CEL-Seq2 uniquely suited to single-cell RNA-Seq analysis in terms of economics, resolution, and ease of use.

X Demographics

X Demographics

The data shown below were collected from the profiles of 28 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 1,181 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 7 <1%
Sweden 3 <1%
United Kingdom 3 <1%
Netherlands 2 <1%
Denmark 2 <1%
Japan 2 <1%
Australia 1 <1%
South Africa 1 <1%
Israel 1 <1%
Other 6 <1%
Unknown 1153 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 281 24%
Researcher 205 17%
Student > Master 137 12%
Student > Bachelor 104 9%
Student > Postgraduate 53 4%
Other 140 12%
Unknown 261 22%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 380 32%
Agricultural and Biological Sciences 263 22%
Medicine and Dentistry 64 5%
Immunology and Microbiology 44 4%
Neuroscience 41 3%
Other 107 9%
Unknown 282 24%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 55. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 April 2024.
All research outputs
#793,413
of 25,784,004 outputs
Outputs from Genome Biology
#516
of 4,517 outputs
Outputs of similar age
#13,813
of 313,549 outputs
Outputs of similar age from Genome Biology
#10
of 80 outputs
Altmetric has tracked 25,784,004 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,517 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 27.6. This one has done well, scoring higher than 88% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 313,549 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 95% of its contemporaries.
We're also able to compare this research output to 80 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 87% of its contemporaries.