Title |
Molecular identification and antigenic characterization of a merozoite surface antigen and a secreted antigen of Babesia canis (BcMSA1 and BcSA1)
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Published in |
Parasites & Vectors, May 2016
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DOI | 10.1186/s13071-016-1518-1 |
Pubmed ID | |
Authors |
Mo Zhou, Shinuo Cao, Yuzi Luo, Mingming Liu, Guanbo Wang, Paul Franck Adjou Moumouni, Charoonluk Jirapattharasate, Aiko Iguchi, Patrick Vudriko, Mohamad Alaa Terkawi, Mario Löwenstein, Angela Kern, Yoshifumi Nishikawa, Hiroshi Suzuki, Ikuo Igarashi, Xuenan Xuan |
Abstract |
Babesia canis is an apicomplexan tick-transmitted hemoprotozoan responsible for causing canine babesiosis in Europe and west Asia. Despite its importance, there is no known rapid diagnostic kit detection of B. canis infection in dogs. The present study identified two novel antigens of B. canis and used the recombinant antigens to establish a rapid, specific and sensitive serodiagnostic technique for detection of B. canis infection. A complementary DNA (cDNA) expression library was constructed from the mRNA of B. canis and immunoscreened using B. canis-infected dog sera. The cDNAs encoding a merozoite surface antigen and a secreted antigen protein were identified and designated as BcMSA1 and BcSA1, respectively. The recombinant BcMSA1 and BcSA1 (rBcMSA1 and rBcSA1) expressed in Escherichia coli were purified and injected into mice for production of anti-sera. The native proteins were characterized by Western blot analysis and immunofluorescence. Furthermore, indirect enzyme-linked immunosorbent assays (iELISA) and rapid immunochromatographic tests (ICT) based on rBcMSA1 or rBcSA1 were established and evaluated to test specific antibodies in consecutive plasma samples from two B. canis-infected dogs. Antiserum raised against rBcMSA1 and rBcSA1 recognized the 39 kDa and 44 kDa native proteins by Western blot analysis, respectively. In addition, immunofluorescence and confocal microscopic observations revealed that BcMSA1 was found on the surface of parasites. However, BcSA1 localized in the matrix of the merozoites. The ELISA and ICT based on rBcMSA1 or rBcSA1 could detect specific antibodies in consecutive plasma samples from two B. canis-infected dogs. They showed no cross-reactions against the serum samples collected from dogs experimentally infected with closely related parasites. Taken together, the current results indicated that the rBcMSA1 and rBcSA1 are promising serodiagnostic antigens for developing iELISA and ICT to detect B. canis infection. To our knowledge, this study is the first to report BcMSA1 and BcSA1 as potential antigenic proteins for serodiagnosis of B. canis infection in dogs. |
X Demographics
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Unknown | 3 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 67% |
Science communicators (journalists, bloggers, editors) | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Uganda | 1 | 3% |
Unknown | 38 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 7 | 18% |
Student > Ph. D. Student | 6 | 15% |
Student > Master | 4 | 10% |
Student > Doctoral Student | 3 | 8% |
Professor | 3 | 8% |
Other | 6 | 15% |
Unknown | 10 | 26% |
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Veterinary Science and Veterinary Medicine | 11 | 28% |
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Biochemistry, Genetics and Molecular Biology | 5 | 13% |
Immunology and Microbiology | 5 | 13% |
Medicine and Dentistry | 2 | 5% |
Other | 1 | 3% |
Unknown | 9 | 23% |