Title |
GMO detection using a bioluminescent real time reporter (BART) of loop mediated isothermal amplification (LAMP) suitable for field use
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Published in |
BMC Biotechnology, April 2012
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DOI | 10.1186/1472-6750-12-15 |
Pubmed ID | |
Authors |
Guy Kiddle, Patrick Hardinge, Neil Buttigieg, Olga Gandelman, Clint Pereira, Cathal J McElgunn, Manuela Rizzoli, Rebecca Jackson, Nigel Appleton, Cathy Moore, Laurence C Tisi, James AH Murray |
Abstract |
There is an increasing need for quantitative technologies suitable for molecular detection in a variety of settings for applications including food traceability and monitoring of genetically modified (GM) crops and their products through the food processing chain. Conventional molecular diagnostics utilising real-time polymerase chain reaction (RT-PCR) and fluorescence-based determination of amplification require temperature cycling and relatively complex optics. In contrast, isothermal amplification coupled to a bioluminescent output produced in real-time (BART) occurs at a constant temperature and only requires a simple light detection and integration device. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 3 | 33% |
Spain | 2 | 22% |
India | 1 | 11% |
Chile | 1 | 11% |
Unknown | 2 | 22% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 7 | 78% |
Science communicators (journalists, bloggers, editors) | 1 | 11% |
Scientists | 1 | 11% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 2 | 2% |
France | 1 | <1% |
Italy | 1 | <1% |
Canada | 1 | <1% |
Argentina | 1 | <1% |
Spain | 1 | <1% |
Poland | 1 | <1% |
Unknown | 106 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 33 | 29% |
Student > Ph. D. Student | 21 | 18% |
Student > Master | 18 | 16% |
Student > Bachelor | 8 | 7% |
Other | 4 | 4% |
Other | 12 | 11% |
Unknown | 18 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 35 | 31% |
Biochemistry, Genetics and Molecular Biology | 27 | 24% |
Engineering | 10 | 9% |
Chemistry | 7 | 6% |
Environmental Science | 6 | 5% |
Other | 10 | 9% |
Unknown | 19 | 17% |