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Quantitative analysis of waterfowl parvoviruses in geese and Muscovy ducks by real-time polymerase chain reaction: correlation between age, clinical symptoms and DNA copy number of waterfowl…

Overview of attention for article published in BMC Veterinary Research, March 2012
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Title
Quantitative analysis of waterfowl parvoviruses in geese and Muscovy ducks by real-time polymerase chain reaction: correlation between age, clinical symptoms and DNA copy number of waterfowl parvoviruses
Published in
BMC Veterinary Research, March 2012
DOI 10.1186/1746-6148-8-29
Pubmed ID
Authors

Grzegorz Woźniakowski, Elżbieta Samorek-Salamonowicz, Wojciech Kozdruń

Abstract

Waterfowl parvoviruses cause serious loss in geese and ducks production. Goose parvovirus (GPV) is infectious for geese and ducks while Muscovy duck parvovirus (MDPV) infects Muscovy ducks only. So far, for these viruses' sensitive detection polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP) were applied. However, there was no molecular biology method for both waterfowl parvoviruses detection and quantification which could unify the laboratory procedures. The level of GPV and MDPV replication and distribution plays a significant role in the parvoviral infection progress and is strictly correlated to clinical symptoms. Meanwhile, experiments conducted previously on GPV distribution in geese, performed as animal trial, did not involve epidemiological data from the disease field cases. The study on the correlation between age, clinical symptoms and viral DNA copy number may be benefitable in understanding the GPV and MDPV infection. Such data may also aid in determination of the stage and severity of the infection with parvoviruses. Therefore the aim of this study was to develop quantitative real-time PCR for parallel detection of GPV and MDPV in geese and Muscovy ducks and to determine the correlation between the age of the infected birds, clinical symptoms and DNA copy number for the estimation of the disease stage or severity.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 5%
Germany 1 5%
Unknown 19 90%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 29%
Student > Doctoral Student 3 14%
Student > Bachelor 2 10%
Professor 2 10%
Student > Ph. D. Student 2 10%
Other 2 10%
Unknown 4 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 38%
Veterinary Science and Veterinary Medicine 3 14%
Biochemistry, Genetics and Molecular Biology 3 14%
Unspecified 1 5%
Environmental Science 1 5%
Other 0 0%
Unknown 5 24%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 May 2012.
All research outputs
#20,656,820
of 25,374,647 outputs
Outputs from BMC Veterinary Research
#2,106
of 3,298 outputs
Outputs of similar age
#132,350
of 169,065 outputs
Outputs of similar age from BMC Veterinary Research
#14
of 18 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,298 research outputs from this source. They receive a mean Attention Score of 4.3. This one is in the 20th percentile – i.e., 20% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 169,065 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 9th percentile – i.e., 9% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 18 others from the same source and published within six weeks on either side of this one. This one is in the 11th percentile – i.e., 11% of its contemporaries scored the same or lower than it.