Title |
Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures
|
---|---|
Published in |
Microbial Cell Factories, May 2012
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DOI | 10.1186/1475-2859-11-57 |
Pubmed ID | |
Authors |
Joel Jordà, Paula Jouhten, Elena Cámara, Hannu Maaheimo, Joan Albiol, Pau Ferrer |
Abstract |
The methylotrophic yeast Pichia pastoris has emerged as one of the most promising yeast hosts for the production of heterologous proteins. Mixed feeds of methanol and a multicarbon source instead of methanol as sole carbon source have been shown to improve product productivities and alleviate metabolic burden derived from protein production. Nevertheless, systematic quantitative studies on the relationships between the central metabolism and recombinant protein production in P. pastoris are still rather limited, particularly when growing this yeast on mixed carbon sources, thus hampering future metabolic network engineering strategies for improved protein production. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Canada | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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France | 2 | <1% |
Spain | 2 | <1% |
Germany | 1 | <1% |
Vietnam | 1 | <1% |
Austria | 1 | <1% |
Brazil | 1 | <1% |
Chile | 1 | <1% |
United Kingdom | 1 | <1% |
India | 1 | <1% |
Other | 2 | <1% |
Unknown | 196 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 56 | 27% |
Researcher | 30 | 14% |
Student > Master | 24 | 11% |
Student > Bachelor | 20 | 10% |
Student > Postgraduate | 12 | 6% |
Other | 33 | 16% |
Unknown | 34 | 16% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 51 | 24% |
Engineering | 20 | 10% |
Chemical Engineering | 9 | 4% |
Chemistry | 6 | 3% |
Other | 14 | 7% |
Unknown | 42 | 20% |